SEMA7A antibody

Catalog No. ABIN110773

Product Details anti-SEMA7A Antibody

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SEMA7A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity: The antibody reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored glycoprotein expressed on various cell types including erythrocytes, lymphoblasts, at low levels it is present on circulating lymphocytes.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ion exchange chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: HPB-ALL human T cell line

Clone: MEM-150

Isotype: IgM

Application Details

Application Notes: Flow Cytometry. Immunoprecipitation. Western Blotting under non reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for SEMA7A

Target: SEMA7A (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A))

Alternative Name: CD108 / SEMA7A (SEMA7A Products)

Synonyms: 2900057C09Rik antibody, CDw108 antibody, H-Sema-L antibody, M-Sema-L antibody, Semal antibody, CD108 antibody, H-SEMA-K1 antibody, JMH antibody, SEMAK1 antibody, SEMAL antibody, sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A antibody, semaphorin 7A (John Milton Hagen blood group) antibody, Sema7a antibody, SEMA7A antibody

Background: CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors plexin C1 and b1 integrins.Synonyms: JMH blood group antigen, John-Milton-Hargen human blood group Ag, SEMAL, Semaphorin-7A, Semaphorin-K1, Semaphorin-L

Gene ID: 8482

NCBI Accession: NP_001139501

UniProt: O75326

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size