Sca-1/Ly-6A/E antibody

Catalog No. ABIN1108075

Product Details anti-Sca-1/Ly-6A/E Antibody

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sca-1/Ly-6A/E antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with Sca-1.

Cross-Reactivity (Details): Species reactivity (tested):Mouse

Purification: Protein G agarose

Immunogen: Mouse Sca-1 transfected LO cells

Clone: 238B

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for Sca-1/Ly-6A/E

Target: Sca-1/Ly-6A/E (Ly6a) (Lymphocyte Antigen 6 Complex, Locus A (Ly6a))

Alternative Name: Ly6A/E / SCA1 (Ly6a Products)

Synonyms: Ly-6A.2 antibody, Ly-6A/E antibody, Ly-6E.1 antibody, Sca-1 antibody, Sca1 antibody, TAP antibody, lymphocyte antigen 6 complex, locus A antibody, Ly6a antibody

Background: Sca-1 is a member of the Ly-6 antigen family which molecular mass of 8 kDa under nonreducing conditions and of 18 kDa under reducing conditions. Mouse hematopoietic stem cell expresses low levels of Thy-1 antigen (Thy-1lo) and to be lineage-negative (Lin-), not express markers characteristic of B cells (B220), granulocytes (Gr-1), myelomonocytic cells (Mac-1) and T lymphocytes (CD4 and CD8). Recently, new monoclonal antibody, anti-Sca-1, was used to purify stem cells from the Thy-1lo, Lin- bone marrow subpopulation. Thy-1lo, Lin-, Sca-1+ (but not the Thy-1lo, Lin-, Sca-1-) population of bone marrow cells are highly purified pluripotent stem cells. They read out with nearly unit efficiency in assays for primitive myeloerythroid and thymic progenitor, and have a capability to admit lethally irradiated mouse to survive and be restored in all blood-cell lineages. The Thy-1lo, Lin-, Sca-1+ subpopulation thought to have all stem cells present in the bone marrow.Synonyms: Ly-6A.2/Ly-6E.1, Lymphocyte antigen 6A-2/6E-1, SCA-1, Stem cell antigen 1, T-cell-activating protein, TAP

Gene ID: 110454

NCBI Accession: NP_034868

UniProt: P05533

Pathways: Sensory Perception of Sound, Activated T Cell Proliferation