MICA antibody

Catalog No. ABIN1108244

Product Details anti-MICA Antibody

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MICA antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Specificity: This antibody reacts with MICA/B. The epitope was mapped to the helical surfaces of the MICα1α2 platform domain.

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein A agarose

Immunogen: MICA*01, MICA*04 and MICB*02 transfected P815 cells

Clone: BAMO1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 50 % Glycerol, pH 7.2, None

Preservative: Without preservative

Storage: -20 °C

Storage Comment: This antibody solution is stable for one year from the date of purchase when stored at -20 °C.

Expiry Date: 12 months

Target Details for MICA

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Alternative Name: MICA (MICA Products)

Synonyms: MIC-A antibody, PERB11.1 antibody, MHC class I polypeptide-related sequence A antibody, MICA antibody

Background: MICA and MICB (Major Histocompatibility Complex class I Chain-related gene A and gene B) bind to the activating immunoreceptor NKG2D. NKG2D is expressed on NK (Natural Killer) cells, NKT cells, γδT cells and CD8+αβT cells. Recognition of MICA and MICB by NKG2D is involved in tumor surveillance, immune responses to viral infections and autoimmune diseases. MICA and MICB are transmembrane glycoproteins that are distantly related to the MIC proteins, and they possess three extra-cellular Ig-like domains. And thus, MICA and MICB are closely related but are functionally indistinguishable. MICA and MICB molecules are highly glycosylated, and are detected as a smear band ranging from 65-75 kDa. It is reported that MICA and MICB are highly expressed in variant tumor cells, whereas normal cells express little. Tumor cells have been shown to shed and release MIC molecules from the cell surface. Therefore determination of soluble MIC (sMIC) levels provides valuable information for cancer staging, andsMIC in serum seems to be an indicator for systemic manifestation of malignancy rather than for local tumor extent.Synonyms: MHC class I polypeptide-related sequence A, MHC class I polypeptide-related sequence B, MIC-A, MIC-B, PERB11.1, PERB11.2

Gene ID: 100507436

NCBI Accession: NP_000238

UniProt: Q29983

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis