PARP4 antibody (AA 408-611)

Catalog No. ABIN1108588

Product Details anti-PARP4 Antibody

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Binding Specificity: AA 408-611

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PARP4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This Monoclonal antobofdy P193-4 reacts with an internal epitope (amino acids 491-494, HPGE) of the minor vault protein (P193 or VPARP), which is overexpressed in various Human non-P-glycoprotein MDR tumor cell lines, accordingly to an increase in the number of vault particles.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Immunogen: E. coli lysate transformed with pET28a(+) expression vector containing amino acids 408-611 of the p193 cDNA.

Clone: P193-4

Isotype: IgG2b

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg IgG/ml

Buffer: 0.09 % Sodium Azide 9 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PARP4

Target: PARP4 (Poly (ADP-Ribose) Polymerase Family, Member 4 (PARP4))

Alternative Name: PARP4 (PARP4 Products)

Synonyms: ADPRTL1 antibody, ARTD4 antibody, PARP-4 antibody, PARPL antibody, PH5P antibody, VAULT3 antibody, VPARP antibody, VWA5C antibody, p193 antibody, Adprtl1 antibody, C030027K23Rik antibody, E230037B21Rik antibody, Gm743 antibody, poly(ADP-ribose) polymerase family member 4 antibody, poly (ADP-ribose) polymerase family, member 4 antibody, PARP4 antibody, Parp4 antibody

Background: PARP-1 is a nuclear protein that is specifically cleaved by caspase-3 and caspase-6, but not by caspase-1, into a signature apoptotic fragment. PARP-2 and PARP-3 interact with PARP-1. PARP-4, also designated vault poly(ADP-ribose) polymerase (VPARP) and ADP-ribotransferase-like 1 (ADPRTL1), associates with the major vault protein (MVP) and telomerase-associated protein 1 (TEP1) to form vaults, barrel-shaped cytoplasmic ribonucleoprotein particles. PARP-4 localizes mainly to the cytoplasm but is also found in the nucleus. The PARP-4 protein is expressed widely, with highest levels observed in the kidney, and is also detected in skeletal muscle, heart, leukocytes, placenta, lung, liver, spleen, and pancreas. PARP-4 contains a PARP (ADPRT)-like catalytic domain, a C-terminal MVP-interacting domain, a domain with two sequences similar to inter-alpha-trypsin inhibitor, and an N-terminal BRCA1 C-terminus (BRCT) domain, which may be involved in protein-protein interactions.Synonyms: 193 kDa vault protein, ADPRTL1, KIAA0177, PARP-4, PARP-related/IalphaI-related H5/proline-rich, PARPL, Poly [ADP-ribose] polymerase 4, VPARP, Vault poly(ADP-ribose) polymerase

Gene ID: 143

NCBI Accession: NP_006428

UniProt: Q9UKK3