PRTN3 antibody

Catalog No. ABIN1108719

Product Details anti-PRTN3 Antibody

Target: PRTN3 (Proteinase 3 (PRTN3))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRTN3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Protein G

Immunogen: A crude granule extract

Clone: PR3-G2

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.02 % sodium azide, 0.1 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for PRTN3

Target: PRTN3 (Proteinase 3 (PRTN3))

Alternative Name: PR3 / C-ANCA (PRTN3 Products)

Synonyms: ela2 antibody, MGC132250 antibody, ACPA antibody, AGP7 antibody, C-ANCA antibody, CANCA antibody, MBN antibody, MBT antibody, NP-4 antibody, NP4 antibody, P29 antibody, PR-3 antibody, PR3 antibody, mPR3 antibody, proteinase 3 L homeolog antibody, proteinase 3 antibody, prtn3.L antibody, PRTN3 antibody, Prtn3 antibody

Background: PR3 is a major antigen recognized by autoantibodies directed against cytoplasmic proteins of neutrophilic granulocytes and monocytes (called anti-neutrophil cytoplasmic autoantibodies (ANCA)). ANCA are able to activate primed neutrophils to produce oxygen radicals and release lytic enzymes, including PR3. Proteinase 3 (PR3) was identified as the target antigen of ANCA in Wegener's granulomatosis (WG). ANCA directed against PR3 (PR3-ANCA) can interfere with the binding of PR3 to its physiological inhibitor alpha1-antitrypsin (alpha1-AT) and with the proteolytic activity of PR3. At the site of inflammation, PR3 can cleave the PR3-ANCA complex between these inhibiting ANCA and PR3 itself, leaving active PR3. Autoantibodies to PR3 are potent activators of the 5-lipoxygenase pathway in primed human neutrophils. Extracellular free arachidonic acid, as present at an inflammatory focus, synergizes with such autoantibodies to evoke full-blown lipid mediator generation, granule secretion and respiratory burst. Proteinase 3 (PR3) is a neutral serine proteinase, which is localized in the azurophilic granules of neutrophils and in granules of monocytes and can be detected in the membrane of secretory vesicles. PR3 degrades a number of extracellular matrix proteins such as elastin and inactivates human C1 inhibitor. Membrane-associated PR3 is also able to activate caspase-3 without triggering apoptosis of neutrophils, which is possibly a neutrophil survival mechanism. In addition, PR3 is involved in myeloid differentiation and is, therefore, also called myeloblastin.Synonyms: AGP7, Leukocyte proteinase 3, MBN, Myeloblastin, NP-4, Neutrophil proteinase 4, P29, PR-3, PRTN3, Proteinase 3, Wegener autoantigen

UniProt: P24158