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Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN1109482, Supplier: Log in to see
Antigen
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
797
291
244
207
121
118
103
79
78
43
40
32
31
27
26
7
5
4
3
2
2
1
1
1
1
1
1
Host
Mouse
522
268
30
12
12
7
5
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
28
26
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
666
294
289
288
240
227
219
131
104
23
15
11
8
7
5
3
3
3
3
1
1
1
Supplier
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Clone 9E7E7
Isotype IgG1
Specificity Recognizes Vimentin
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Ascites
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.Synonyms: VIM
Gene ID 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 0.03 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Supplier Images
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN1109482) anti-Vimentin (VIM) antibody
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN1109482) anti-Vimentin (VIM) antibody (Image 2)
Background publications Seshadri, Raymond, Leong, Horsfall, McCaul: "Vimentin expression is not associated with poor prognosis in breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 67, Issue 3, pp. 353-6, 1996 (PubMed).

Essa, el Tatawi, Hamdi, Arafa: "Vimentin expression in different types of breast carcinoma immunohistochemical study." in: Journal of the Egyptian Society of Parasitology, Vol. 26, Issue 2, pp. 433-42, 1996 (PubMed).

Chu, Seftor, Romer, Hendrix: "Experimental coexpression of vimentin and keratin intermediate filaments in human melanoma cells augments motility." in: The American journal of pathology, Vol. 148, Issue 1, pp. 63-9, 1996 (PubMed).