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Zinc Finger and BTB Domain Containing 4 (ZBTB4) antibody

Details for Product No. ABIN1109539, Supplier: Log in to see
Antigen
  • 2310026P19Rik
  • 9230111I22Rik
  • KAISO-L1
  • mKIAA1538
  • RP23-422L16.16
  • ZNF903
Reactivity
Human
48
20
14
1
Host
Rabbit
48
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
35
22
11
8
3
2
1
1
Supplier
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Immunogen Synthetic peptide derived from an internal domain of Human ZBTB4
Specificity This antibody reacts with 105 kDa ZBTB4 protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein A
Alternative Name ZBTB4 (ZBTB4 Antibody Abstract)
Background The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions, this degradation increases with DNA damage.Synonyms: KAISO-L1, KAISO-like zinc finger protein 1, KIAA1538, Zinc finger and BTB domain-containing protein 4
Gene ID 57659
NCBI Accession NP_001122305
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Reconstitution Restore in distilled water.
Buffer 0.1 M Tris, 0.1 M Glycine, 2 % Sucrose, None
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at -20 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Weber, Marquardt, Elzi, Forster, Starke, Glaum, Yamada, Defossez, Delrow, Eisenman, Christiansen, Eilers: "Zbtb4 represses transcription of P21CIP1 and controls the cellular response to p53 activation." in: The EMBO journal, Vol. 27, Issue 11, pp. 1563-74, 2008

Nguyen, Yamada, Schultz, Defossez: "Assessment of sera for chromatin-immunoprecipitation." in: BioTechniques, Vol. 44, Issue 1, pp. 66, 68, 2008