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Cyclin D1 antibody (CCND1)

Details for Product anti-CCND1 Antibody No. ABIN111000, Supplier: Login to see New
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Dog (Canine), Guinea Pig, Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus)
(394), (139), (127), (18), (14), (13), (11), (9), (8), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
(326), (126), (1), (1)
Clonality (Clone)
Monoclonal ()
This Cyclin D1 antibody is un-conjugated
(21), (17), (5), (5), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS)
(353), (165), (96), (92), (80), (70), (44), (43), (42), (13), (11), (8), (7), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 7 references available
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Quantity 50 μg
Shipping to United States ( )
Immunogen Human recombinant full-length cyclin D1 protein
Clone DCS-6
Isotype IgG2a
Specificity DCS-6 reacts specifically with cyclin D1 protein present predominantly in G1 phase of cell cycle. Does not cross-react with D2 or D3 cyclins (the closest relatives within the cyclin family). DCS-6 is especially useful in tumor diagnosis: it can be used as fast method to identify cases with 11q13 amplifications. Reacts with MCF-7, MDA-MB-134 (breast cancer cell lines), UMSC22 (squamous cell carcinoma cell line), U-2-OS (osteosarcoma cell line) RT 112, A 431, HT 29 (for additional cell lines and their reaction patterns see ref. 1).
Purification Protein A affinity chromatography
Alternative Name Cyclin D1 (CCND1 Antibody Abstract)
Background Cyclin D1 gene is identical to the BCL-1 and the PRAD-1 oncogene and is part of the chromosome 11q13 amplicon which is amplified in several common tumor types like breast, lung, esophagus, urinary bladder and head and neck carcinoma.
Alternate names: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1
Gene ID 595
NCBI Accession NP_444284
UniProt P24385
Research Area Cancer, Cell Cycle
Pathways PI3K-Akt Signaling, Cell Division Cycle
Application Notes Immunostaining of frozen tissue and culture cells (5 ug/ml). Immunoblotting (Western Blot): 1-2 ug/ml for 2hrs at RT. Immunoprecipitation (Co-precipitates Cdk4)(Native and denatured): Use Protein A andAbat 2g/mg protein lysate)Microinjection/in vitro neutralization of cyclin D1 function (use ABIN181731). Flow cytometry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Reconstitute with 1.0 mL dist. water.
Buffer PBS pH 7.4 with 0.5% BSA and 0.09% sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in small aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Reichelt, Magin: "Hyperproliferation, induction of c-Myc and 14-3-3sigma, but no cell fragility in keratin-10-null mice." in: Journal of cell science, Vol. 115, Issue Pt 13, pp. 2639-50, 2002 (PubMed).

Bartkova, Lukas, Strauss et al.: "Cell cycle-related variation and tissue-restricted expression of human cyclin D1 protein." in: The Journal of pathology, Vol. 172, Issue 3, pp. 237-45, 1994 (PubMed).

Lukas, Müller, Bartkova et al.: "DNA tumor virus oncoproteins and retinoblastoma gene mutations share the ability to relieve the cell's requirement for cyclin D1 function in G1." in: The Journal of cell biology, Vol. 125, Issue 3, pp. 625-38, 1994 (PubMed).

Bartkova, Lukas, Müller et al.: "Cyclin D1 protein expression and function in human breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 57, Issue 3, pp. 353-61, 1994 (PubMed).

Lukas, Bartek, Strauss: "Efficient transfer of antibodies into mammalian cells by electroporation." in: Journal of immunological methods, Vol. 170, Issue 2, pp. 255-9, 1994 (PubMed).

Lukas, Pagano, Staskova et al.: "Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines." in: Oncogene, Vol. 9, Issue 3, pp. 707-18, 1994 (PubMed).

Lukas, Jadayel, Bartkova et al.: "BCL-1/cyclin D1 oncoprotein oscillates and subverts the G1 phase control in B-cell neoplasms carrying the t(11;14) translocation." in: Oncogene, Vol. 9, Issue 8, pp. 2159-67, 1994 (PubMed).

Catalog No. ABIN111000

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