Goat anti-Rat IgG (Heavy & Light Chain) Antibody (DyLight 550)

Catalog No. ABIN1110480

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: DyLight 550

Application: Immunoassay (IA)

Specificity: Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG and light chains on all rat immunoglobulins.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: Goat anti-Rat IgG (H&L), F(ab) '2 fragment - DyLight® 550 Conjugated, is a secondary antibody conjugated to DyLight® 550, which binds to all rat IgG (H&L), F(ab) fragment in immunological assays. DyLight® 550 has Amax = 562 nm, Emax = 576 nm. Antibodies are are affinity purified using solid phase rat IgG (H&L).

Purification: Affinity purification

Immunogen: Purified Rat IgG, whole molecule

Application Details

Application Notes: 1:20 - 1:2,000 for most applications

Comment:

Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin mouse serum proteins IgG from human or mouse.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody