Donkey anti-Rat IgG (Heavy & Light Chain) Antibody (DyLight 594)

Catalog No. ABIN1110590

Product Details

Target: IgG

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Donkey

Clonality: Polyclonal

Conjugate: DyLight 594

Application: Immunoassay (IA)

Specificity: Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on rat IgG and light chains on all rat immunoglobulins.

Cross-Reactivity: Sheep (Ovine), Chicken, Goat, Guinea Pig, Hamster, Horse (Equine), Human, Mouse (Murine), Rabbit

Characteristics: Donkey anti-Rat IgG (H&L), DyLight594 Conjugate, is a secondary antibody conjugated to DyLight 594, which binds to rat IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase rat IgG.

Purification: Affinity purification

Immunogen: Purified rat IgG, whole molecule

Application Details

Application Notes: 1:20 - 1:2,000 for most applications

Comment:

Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on rat IgG light chains on all rat immunoglobulins.No reactivity is observed to: non-immunoglobulin rat serum proteins IgG from bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rabbit or sheep.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.

Storage: 4 °C/-20 °C

Storage Comment: Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody