Donkey anti-Sheep IgG (Heavy & Light Chain) Antibody (DyLight 594)
IA
Polyclonal
DyLight 594
-
- Target See all IgG products
- IgG
-
Binding Specificity
- Heavy & Light Chain
-
Reactivity
- Sheep
-
Host
- Donkey
-
Clonality
- Polyclonal
-
Conjugate
- DyLight 594
-
Application
- Immunoassay (IA)
- Specificity
- Based on immunoelectrophoresis, this antibody reacts with: heavy (γ) chains on sheep IgG and light chains on all sheep immunoglobulins.
- Characteristics
- Donkey anti-Sheep IgG (H&L), DyLight594 Conjugate is a secondary antibody conjugated to DyLight 594, which binds to all sheep IgG (H&L) in immunological assays. DyLight 550 has Amax = 593 nm, Emax = 618 nm. Antibodies are are affinity purified using solid phase sheep IgG.
- Purification
- Affinity purification
- Immunogen
- Purified sheep IgG, whole molecule
-
-
- Application Notes
- 1:20 - 1:2,000 for most applications
- Comment
-
Based on immunoelectrophoresis, this antibody reacts with: heavy (gamma) chains on sheep IgG light chains on all sheep immunoglobulins.No reactivity is observed to: non-immunoglobulin sheep serum proteins.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- Handling Advice
-
Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
-
You are here:
