26S Proteasome (p32 subunit) antibody

Antigen: 26S Proteasome (p32 subunit)

Clonality: Monoclonal (P32(26S-161))

Host: Mouse

Reactivity: Human, Rat (Rattus), Xenopus laevis

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN111069

Additional Information

Characteristics: Synonyms: Proteasome26S

Immunogen: 26S complexes of Xenopus laevis oocytes.

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: P32(26S-161)

Specificity: Recognizes the 20S subcomplex within the 26S heterooligomeric protein complex and thefree cytosolic form of 20S cylinder particles, the epitope is resistant to formaldehydefixation (up to 4%). Antigen Recognized in Species and Cultured Cell Lines (tested so far): Xenopus laevis,human, XLKE-A6 (Xenopus) PLC, MCF-7, A431, CaCo (human), 3T3 (mouse), RV (rat), Ptk2(rat kangaroo)

Application Details

Application Notes: Western Blot: 1: 1000 when using the ECL method. Immunofluorescence microscopy: Ready-to-use, 20 - 30 min at RT, (longer incubation mayresult in loss of soluble antigen). P32 (26S-161) is not suitable for immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: contains 0.09% NaN3

Storage: Store the antibody undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Publications: Peters: "Proteasomes: protein degradation machines of the cell." in: Trends in biochemical sciences, Vol. 19, Issue 9, pp. 377-82, 1995 (PubMed).

Peters, Franke, Kleinschmidt: "Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7709-18, 1994 (PubMed).