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26S Proteasome antibody

Details for Product No. ABIN111069
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Antigen
Reactivity
Human, Rat (Rattus), Xenopus laevis
(4), (2), (1)
Host
Mouse
(5)
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(5), (3), (1), (1)
Pubmed 2 references available
Quantity 5 mL
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Catalog No. ABIN111069
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Immunogen 26S complexes of Xenopus laevis oocytes.
Clone P32(26S-161)
Isotype IgG1
Specificity Recognizes the 20S subcomplex within the 26S heterooligomeric protein complex and the free cytosolic form of 20S cylinder particles. The epitope is resistant to formaldehyde fixation (up to 4%). Antigen Recognized in Species and Cultured Cell Lines (tested so far): Xenopus laevis, Human, XLKE-A6 (Xenopus) PLC, MCF-7, A431, CaCo (Human), 3T3 (Mouse), RV (Rat), Ptk2 (Rat kangaroo).
Purification Supernatant
Background The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.
Alternate names: Proteasome26S
Application Notes Western Blot: 1: 1000 when using the ECL method. Immunofluorescence microscopy: Ready-to-use, 20 - 30 min at RT, (longer incubation mayresult in loss of soluble antigen). P32 (26S-161) is not suitable for immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Shelf life one year from despatch.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for up to one month or at -20°C for longer.
Background publications Peters, Franke, Kleinschmidt: "Distinct 19 S and 20 S subcomplexes of the 26 S proteasome and their distribution in the nucleus and the cytoplasm." in: The Journal of biological chemistry, Vol. 269, Issue 10, pp. 7709-18, 1994 (PubMed).

General Peters: "Proteasomes: protein degradation machines of the cell." in: Trends in biochemical sciences, Vol. 19, Issue 9, pp. 377-82, 1995 (PubMed).

Validation Images
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