Human beta 2-Microglobulin (MW 12 kDa). Recognizes the HLA-beta 2-Microglobulin complex, free or associated with the membrane. Does not react with other species. The exact epitope for the antibody has not been mapped.
Purification of the HLA antigens associated with beta 2-Microglobulin. Fluorescence Microscopy or Flow Cytometry: 2 μg/5x10e5 cells/test. Immunohistochemistry (Frozen Sections or Cell Smears): 1/25-1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water
Concentration
0.2 mg/mL (prior to lyophilization)
Buffer
PBS, None, 1 mg/mL BSA
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Following reconstitution store the antibody at -20 °C.
Beta-2 Microglobulin non-covalently associates with the 44 kDa alpha chain to form the HLA Class I antigen complex. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.Synonyms: B2M, Beta 2 microglobulin, Beta-2 microglobulin