Goat anti-Human IgE (Chain epsilon) Antibody (DyLight 650)
IA
Polyclonal
DyLight 650
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- Target See all IgE products
- IgE
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Binding Specificity
- Chain epsilon
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- DyLight 650
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Application
- Immunoassay (IA)
- Specificity
- Based on immunoelectrophoresis, this antibody reacts with: heavy (epsilon) chains on human IgE (epsilon chain)
- Characteristics
- Goat anti-human IgE (ε chain) - DyLight® 650 Conjugated is a secondary antibody conjugated to DyLight® 650, which binds to human IgE (ε chain) in immunological assays. DyLight® 650 has Amax = 652 nm, Emax = 672 nm. Antibodies are are affinity purified using solid phase human IgE (ε chain).
- Purification
- Affinity purification
- Immunogen
- purified human IgE (epsilon chain)
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- Application Notes
- 1:20 - 1:2,000 for most applications
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
- Buffer
- 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v).
- Handling Advice
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Product is photosensitive and should be protected from light.
Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store lyophilized material at 2-8°C. Product is stable for 4 weeks at 2-8°C after rehydration.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
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- Target
- IgE
- Abstract
- IgE Products
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