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KRT20 antibody (FITC)

KRT20 Reactivity: Human, Rat, Pig IHC (p), IF, IHC (fro), FACS Host: Mouse Monoclonal KS20-10 FITC
Catalog No. ABIN111170
  • Target See all KRT20 Antibodies
    KRT20 (Keratin 20 (KRT20))
    Reactivity
    • 91
    • 29
    • 27
    • 9
    • 6
    • 2
    • 2
    • 1
    • 1
    Human, Rat, Pig
    Host
    • 62
    • 30
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 52
    • 45
    Monoclonal
    Conjugate
    • 63
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This KRT20 antibody is conjugated to FITC
    Application
    • 68
    • 46
    • 25
    • 15
    • 14
    • 12
    • 11
    • 9
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)
    Specificity
    This Monoclonal antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20, Mr 46 000, also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
    No Cross-Reactivity
    Mouse (Murine)
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Porcine (Pig) and Rat.
    Purification
    Affinity Chromatography
    Immunogen
    Electrophoretically purified Keratin K 20 from Human intestinal mucosa.
    Clone
    KS20-10
    Isotype
    IgG1
    Top Product
    Discover our top product KRT20 Primary Antibody
  • Application Notes
    FACS. Cytological Material. Immunohistochemistry on Frozen Sections. . Immunohistochemistry on Paraffin sections (Only after microwave treatment). Working Dilution: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8 °C.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.2 mg/mL
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Mușină, Zlei, Mentel, Scripcariu, Ștefan, Aniţei, Filip, Radu, Gavrilescu, Panuţa, Buna-Arvinte, Cordun, Predescu, Scripcariu, Huţanu: "Evaluation of circulating tumor cells in colorectal cancer using flow cytometry." in: The Journal of international medical research, Vol. 49, Issue 9, pp. 300060520980215, (2021) (PubMed).

  • Target
    KRT20 (Keratin 20 (KRT20))
    Alternative Name
    Cytokeratin 20 (KRT20 Products)
    Synonyms
    KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody
    Background
    Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
    Gene ID
    54474
    NCBI Accession
    NP_061883
    UniProt
    P35900
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