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Pan Cytokeratin antibody

Details for Product No. ABIN111175
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Antigen
Reactivity
Human
(22), (10), (5), (5), (5), (4), (4), (2), (2), (2), (1), (1), (1)
Host
Mouse
(20), (4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(2), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(22), (16), (12), (12), (9), (5), (2), (1), (1), (1)
Pubmed 4 references available
Quantity 0.5 mL
Shipping to United States (Change)
Availability Discontinued
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Catalog No. ABIN111175
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Immunogen Human keratin isolated from epidermal stratum corneum.
Clone KL1
Isotype IgG1
Specificity This antibody is specific to 56 kD cytokeratin. Immunoblotting analysis revealed that this antibody reacts with cytokeratin 1, 2, 5, 6, 7, 8, 11, 14, 16, 17 and 18. This antibody stains with neoplastic cells of epithelial origin. Non-epithelial cells and skin basal layers cells do not stain with this antibody. Cellular Localization: Cytoplasmic
Purification Purified
Background Alternate names: Cytokeratin pan-reactive, pan Keratin
Application Notes Immunohistochemistry on formalin-fixed paraffin embedded sections. This antibody may be diluted to a titer of 1: 25-1: 50 in an ABC method (30 minutesincubation at room temperature). Proteolytic treatment prior to immunostaining of formalin fixed paraffin embedded tissuesections may enhance staining intensity. Positive Control: SkinOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Pan Cytokeratin antibody Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin Pan antibody.
General Takahashi, Fujita, Okabe et al.: "Immunohistochemical characterization of basal cell adenomas of the salivary gland." in: Pathology, research and practice, Vol. 187, Issue 2-3, pp. 145-56, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Viac, Reano, Brochier et al.: "Reactivity pattern of a monoclonal antikeratin antibody (KL1)." in: The Journal of investigative dermatology, Vol. 81, Issue 4, pp. 351-4, 1983 (PubMed).

Wells, Heryet, Brochier et al.: "The immunocytochemical detection of axillary micrometastases in breast cancer." in: British journal of cancer, Vol. 50, Issue 2, pp. 193-7, 1984 (PubMed).

Validation Images
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