pan Cytokeratin antibody

Antigen: pan Cytokeratin

Clonality: Monoclonal (KL1)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111175

Additional Information

Alternative name:

Immunogen: Human keratin isolated from epidermal stratum corneum.

Cross-Reactivity: Human

Isotype: IgG1

Clone: KL1

Characteristics: Synonyms: pan Keratin, Cytokeratin Pan-reactive

Application Details

Application Notes: Immunohistochemistry on formalin-fixed paraffin embedded sections. This antibody may be diluted to a titer of 1: 25-1: 50 in an ABC method (30 minutesincubation at room temperature). Proteolytic treatment prior to immunostaining of formalin fixed paraffin embedded tissuesections may enhance staining intensity. Positive Control: SkinOther applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Purification: Purified

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Images

Formalin fixed paraffin embedded human lung carcinoma stained with Cytokeratin Pan antibody. ABIN111174 (ABIN111174-05)

Publications

Takahashi, Fujita, Okabe et al.: "Immunohistochemical characterization of basal cell adenomas of the salivary gland." in: Pathology, research and practice, Vol. 187, Issue 2-3, pp. 145-56, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Viac, Reano, Brochier et al.: "Reactivity pattern of a monoclonal antikeratin antibody (KL1)." in: The Journal of investigative dermatology, Vol. 81, Issue 4, pp. 351-4, 1983 (PubMed).

Wells, Heryet, Brochier et al.: "The immunocytochemical detection of axillary micrometastases in breast cancer." in: British journal of cancer, Vol. 50, Issue 2, pp. 193-7, 1984 (PubMed).