Keratin 18 (KRT18) antibody (Biotin)

Antigen: Keratin 18 (KRT18)

Synonyms: K18, CYK18, CK18, Krt1-18, MGC109143, cb83, dapk1, DreK18, sb:cb83, wu:fa13f02, wu:fb36b04, krt18, MGC64569, MGC75922, KRT18, MGC131121

Clonality: Monoclonal (Ks18-04)

Host: Mouse

Reactivity: Cow (Bovine)

Conjugate: Biotin

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN111181

Additional Information

Alternative name: Cytokeratin 18

UniProt: P05783

Immunogen: Cytokeratin 18 of molecular weight 45kDa. Purified from a HeLa cytoskeletal preparation.

Cross-Reactivity: Cow (Bovine), Dog (Canine), Hamster, Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Sheep (Ovine)

Isotype: IgG1

Clone: Ks18-04

Description: Cytokeratin 18 is an acidic keratin which is found primarily in non squamous epithelia andis present in a majority of adenocarcinomas and ductal carcinomas but not in squamouscell carcinomas. Cytokeratin 18 exists in combination with Cytokeratin 8, a basic keratin. Hepatocellular carcinomas have been reportedly defined by the use of antibodies thatrecognize only Cytokeratins 8 and 18.

Characteristics: Synonyms: Cytokeratin-18, CK18, Keratin-18, Keratin 18, KRT18, CYK18, K18, Cell proliferation-inducinggene 46 protein, Keratin type I cytoskeletal 18

Specificity: Ks 18. 04 represents an excellent marker to discriminate simple epithelia from those ofdifferent origin. Tumors specifically detected: all adenocarcinoma, mammary carcinoma,urinary bladder carcinoma, undifferentiated carcinoma, cervix carcinoma, hepatocellularcarcinoma. Polypeptide reacting: Mr 45 000 polypeptide (human keratin K18, formerly alsodesignated cytokeratin 18) of all simple type epithelia and basal cells of many squamous,nonepidermal epithelia. Species: Human, Mouse, Rat, Bovine, Pig, Dog, Hamster,Sheep, Fish (trout). Others not tested.

Application Details

Application Notes: Immunohistochemistry on frozen and paraffin sections. ELISA. Immunoblotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use only

Publications

Bártek, Vojtĕsek, Stasková et al.: "A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology." in: The Journal of pathology, Vol. 164, Issue 3, pp. 215-24, 1991 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).

Gomi, Yokoyama, Fujimoto et al.: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995 (PubMed).

Bantel, Ruck, Gregor et al.: "Detection of elevated caspase activation and early apoptosis in liver diseases." in: European journal of cell biology, Vol. 80, Issue 3, pp. 230-9, 2001 (PubMed).