GFAP
Reactivity: Human
WB, IHC, IF
Host: Rabbit
Monoclonal
unconjugated
Application Notes
Immunoblotting. Immunofluorescence Microscopy. Immunohistochemistry. Cytological MaterialImmunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. Working Dilutions: 1/10 with PBS, pH 7.4, for Immunohistochemical application. Incubation Time: 1 h ar RT, extended with paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL distilled water
Buffer
PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytesSynonyms: Glial Fibrillary Acidic Protein