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GFAP antibody

GFAP Reactivity: Human, Rat, Mouse, Cow WB, IF, IHC (p), IHC (fro) Host: Mouse Monoclonal GF12-24 unconjugated
Catalog No. ABIN111187
  • Target See all GFAP Antibodies
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    Reactivity
    • 292
    • 171
    • 164
    • 50
    • 48
    • 33
    • 30
    • 11
    • 9
    • 9
    • 7
    • 6
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Cow
    Host
    • 194
    • 138
    • 12
    • 3
    • 2
    • 1
    Mouse
    Clonality
    • 195
    • 155
    Monoclonal
    Conjugate
    • 161
    • 29
    • 25
    • 19
    • 13
    • 9
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    This GFAP antibody is un-conjugated
    Application
    • 283
    • 129
    • 101
    • 82
    • 73
    • 63
    • 44
    • 30
    • 26
    • 26
    • 22
    • 19
    • 13
    • 8
    • 8
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects. Polypeptide Reacting: Mr 50 000 glial filament protein GFP. Tumors Specifically Reacting: Astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas. Tested Reactivities on Cultured Cell Lines: U 333 CG/343MG.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, Bovine, Mouse and Rat.
    Purification
    Protein A Chromatography
    Immunogen
    Intermediate filament cytoskeleton from cultured human glioma cells.
    Clone
    GF12-24
    Isotype
    IgG2a
    Top Product
    Discover our top product GFAP Primary Antibody
  • Application Notes
    Immunoblotting. Immunofluorescence Microscopy. Immunohistochemistry. Cytological MaterialImmunohistochemistry on Frozen Sections and Paraffin-Embedded Sections. Working Dilutions: 1/10 with PBS, pH 7.4, for Immunohistochemical application. Incubation Time: 1 h ar RT, extended with paraffin.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1.0 mL distilled water
    Buffer
    PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody at -20 °C.
  • Target
    GFAP (Glial Fibrillary Acidic Protein (GFAP))
    Alternative Name
    GFAP (GFAP Products)
    Synonyms
    GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody
    Background
    Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytesSynonyms: Glial Fibrillary Acidic Protein
    Gene ID
    2670
    NCBI Accession
    NP_001229305
    UniProt
    P14136
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