POLR2A antibody
| Antigen |
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| Clonality |
Monoclonal (8WG16) |
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Host
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Reactivity
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 Alternatives Cow (Bovine), Human, Mouse (Murine), Xenopus laevis, Yeast (Saccharomyces Cerevisiae)
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Application
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Alternatives Immunofluorescence (IF), Western Blotting (WB), Chromatin Immunoprecipitation (ChiP)
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| Catalog no. |
ABIN111190 |
| Quantity |
0.2 mg (1.0 mg/ml (OD280 nm)) |
| Price |
510.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: RNA polymerase II subunit B1, POLR2, POLRA, RPOL2, RPB1, RPB220, DNA-directed RNApolymerase II subunit RPB1, RNA-directed RNA polymerase II subunit RPB1, DNA-directedRNA polymerase II largest subunit, RNA polymerase II 220 kd
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Gene ID
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5430
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Swiss-Prot
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P24928
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Immunogen
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Purified wheat germ RNA polymerase II
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Isotype
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IgG2a (Matching secondary antibodies)
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Clone
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8WG16
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Specificity
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Reacts with the highly conserved heptapeptide repeat of the largest subunit of EukaryoticRNA polymerase II. It inhibits promoter-directed transcription, but it does not inhibit elongation in thenonspecific transcription assay. S. cerevisiae. Species: Human, bovine, mouse and xenopus. Others not tested.
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Application Notes
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Suitable for use in Immunofluorescence, Western blot andDetection of unproteolyzed RNA polymerase II. Inhibit specific transcription from class II promoters. As a polyol-responsive antibody it can be used in gentle purifications of RNA polymerase IIfrom wheat germ, calf thymus, and yeast and is likely to purify RNA polymerase II frommost eukaryotic organisms. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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1.0 mg/ml (OD280 nm)
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Buffer
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PBS, pH 7.4 without preservatives.
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Storage
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Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Restrictions
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For Research Use only
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Publications
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Thompson, Aronson, Burgess: "Purification of eukaryotic RNA polymerase II by immunoaffinity chromatography. Elution of active enzyme with protein stabilizing agents from a polyol-responsive monoclonal antibody." in: The Journal of biological chemistry, Vol. 265, Issue 12, pp. 7069-77, 1990 (PubMed).
Thompson, Steinberg, Aronson et al.: "Inhibition of in vivo and in vitro transcription by monoclonal antibodies prepared against wheat germ RNA polymerase II that react with the heptapeptide repeat of eukaryotic RNA polymerase II." in: The Journal of biological chemistry, Vol. 264, Issue 19, pp. 11511-20, 1989 (PubMed).
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Alternatives for antigen "POLR2A", type "Antibodies"
