CD53 antibody (Biotin)

Catalog No. ABIN1112184

Product Details anti-CD53 Antibody (Biotin)

Target: CD53

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD53 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Characteristics: Monoclonal Mouse Anti-Human CD53.The conjugate is provided in liquid form in buffer containing 1% bovine serum albumin (BSA),(Lote: 113K1364/SIGMA) and 0,09% NaN3, pH 7.2.

Clone: HI29

Isotype: IgG1

Application Details

Application Notes: It is recommended for use in flow cytometry. This reagent is effective for direct immunofluorescence staining of human tissue for flow cytometric analysis using 10 µl, 1/100, of the suggested working dilution to label 10^6 cells.

Comment:

BIOTIN (Sigma).

Restrictions: For Research Use only

Handling

Preservative: Sodium azide

Precaution of Use: 1. The device is not intended for clinical use including diagnosis, prognosis, and monitoring of a disease state, and it must not be used in conjunction with patient records or treatment. 2. This product contains Sodium azide (NaN3), a chemical highly toxic in pure form. At product concentrations, though not classified as hazardous, Sodium azide may react with lead and copper plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent metal azide build-up in plumbing. 3. As with any product derived from biological sources, proper handling procedures should be used.

Storage: 4 °C

References for anti-CD53 antibody (Biotin) (ABIN1112184)

Kim, Yoon, Kim, Yook, Kim, Kim, Lee, Paik: "LPS-induced CD53 expression: a protection mechanism against oxidative and radiation stress." in: Molecules and cells, Vol. 17, Issue 1, pp. 125-31, (2004) (PubMed).

Yunta, Rodríguez-Barbero, Arévalo, López-Novoa, Lazo: "Induction of DNA synthesis by ligation of the CD53 tetraspanin antigen in primary cultures of mesangial cells." in: Kidney international, Vol. 63, Issue 2, pp. 534-42, (2003) (PubMed).

Target Details for CD53

Target: CD53

Alternative Name: CD53 (CD53 Products)

Background: The antigen, 32-40kDa, is a single chain glycoprotein on most leucocytes. There is no cross- reactivity with erythrocytes or platelets. The antibody recognises a broad span of leucocytes of both normal and neoplastic plasma cells.