CD2 Molecule (CD2) antibody

Antigen: CD2 Molecule (CD2)

Synonyms: T11, SRBC, FLJ46032, CD2, MGC140284, CD2R, LFA2, OX34, LFA-2, OX-34, Ly37, Ly-37

Clonality: Monoclonal (39C1-5)

Host: Rat

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func), Flow Cytometry (FACS)

Catalog no.: ABIN111249

Additional Information

Alternative name: CD2

UniProt: P06729

Immunogen: Human PHA-stimulated lymphocytic blasts.

Cross-Reactivity: Human

Isotype: IgG2a

Clone: 39C1-5

Description: CD2 is a surface antigen of the human T lymphocyte lineage that is expressed on allperipheral blood T cells. It is one of the earliest T cell markers, being present on more than95% of thymocytes, it is also found on some natural killer cells but not on B lymphocytes. CD2 interacts with lymphocyte function associated antigen (LFA3) and CD48/BCM1 tomediate adhesion between T cells and other cell types. CD2 is implicated in the triggeringof T cells, the cytoplasmic domain is implicated in the signaling function. It is useful for theidentification of lymphomas and leukaemias of T cell origin.

Characteristics: Synonyms: T11/Leu-5, LFA-2, LFA-3 receptor, Erythrocyte receptor, Rosette receptor, SRBC, T-cellsurface antigen CD2, T-cell surface antigen T11/Leu-5

Application Details

Application Notes: Identification of T cells: Characterization of tumoral subsets in Leukemia, T Lymphoma andSezary syndrome Study of T cell functions. Fluorescence Microscopy or Flow Cytometry (2 µg/5x10e5 cells/test). Immunohistochemistry (1/25-1/50): This antibody is only suitable on cryostat sections orcell smears. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml (prior to lyophilization)

Buffer: PBS containing 1 mg/ml BSA as stabilizer and no preservative.Reconstitution: Restore with 1 ml of distilled water.

Preservative: Azide Free

Storage: Store lyophilized product at 2-8°C. Store the reconstituted antibody at 2-8°C for one month (add 0. 09% Sodium Azide) or at-20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Stem Cells, Hematopoietic Progenitors, Hematopoietic Stem Cells, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Dengler, Hoffmann, Knolle et al.: "Structural and functional epitopes of the human adhesion receptor CD58 (LFA-3)." in: European journal of immunology, Vol. 22, Issue 11, pp. 2809-17, 1992 (PubMed).

Olive, Ragueneau, Cerdan et al.: "Anti-CD2 (sheep red blood cell receptor) monoclonal antibodies and T cell activation. I. Pairs of anti-T11.1 and T11.2 (CD2 subgroups) are strongly mitogenic for T cells in presence of 12-O-tetradecanoylphorbol 13-acetate." in: European journal of immunology, Vol. 16, Issue 9, pp. 1063-8, 1986 (PubMed).

Breitmeyer, Daley, Levine et al.: "The T11 (CD2) molecule is functionally linked to the T3/Ti T cell receptor in the majority of T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 9, pp. 2899-905, 1987 (PubMed).

Denning, Dustin, Springer et al.: "Purified lymphocyte function-associated antigen-3 (LFA-3) activates human thymocytes via the CD2 pathway." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 141, Issue 9, pp. 2980-5, 1988 (PubMed).

Meuer, Hussey, Fabbi et al.: "An alternative pathway of T-cell activation: a functional role for the 50 kd T11 sheep erythrocyte receptor protein." in: Cell, Vol. 36, Issue 4, pp. 897-906, 1984 (PubMed).