CD80 (CD80) antibody

Antigen: CD80 (CD80)

Synonyms: LAB7, CD28LG, CD28LG1

Clonality: Monoclonal (MAB104)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (IHC)

Catalog no.: ABIN111250

Additional Information

Alternative name: CD80

UniProt: P33681

Immunogen: Jijoye cells (Human Burkitt Lymphoma cell line)

Cross-Reactivity: Human

Isotype: IgG1

Clone: MAB104

Description: CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules,CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result inenhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated Bcells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines,freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed athigh levels in dendritic cells. It reacts weakly with a small proportion of non activatednormal B cells and with HTLV1 infected T cells. CD80 does not react with peripheralmonocytes, resting and activated normal T cells, T cell lines and T cell clones, nor withmyelomonocytic cell lines.

Characteristics: Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1

Specificity: The antibody reacts with in vitro activated normal B cells. Reacts weakly with a small proportion of non-activated normal B cells. Does not react with peripheral monocytes, resting and activated normal T cells, T-cell linesand T-cell clones. Add. Information: The molecular weight of the recognized antigen is 60 kDa.

Application Details

Application Notes: Flow cytometry. Functional study of B cell subpopulations. Fluorescence microscopy or flow cytometry (2 g/5x10e5 cells/test). Immunohistochemistry (1: 20 to 1: 50, suitable on frozen sections or cell smears). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.2 mg/ml (prior to lyophilization)

Purification: Azide Free

Buffer: PBS containing 1.0 mg/ml BSAReconstitution: Restore with 1.0 ml of distilled water.

Storage: Store lyophilized product at 2-8°C. After reconstitution, aliquot and store at -20°C. Avoid multiple freeze/thaw cycles. Shelf life: one year from despatch. The addition of 0. 1% sodium azide is recommended for storage of the reconstituted formfor up to one month at 2-8°C.

Research Area: Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Valle, Aubry, Durand et al.: "IL-4 and IL-2 upregulate the expression of antigen B7, the B cell counterstructure to T cell CD28: an amplification mechanism for T-B cell interactions." in: International immunology, Vol. 3, Issue 3, pp. 229-35, 1991 (PubMed).

June, Ledbetter, Linsley et al.: "Role of the CD28 receptor in T-cell activation." in: Immunology today, Vol. 11, Issue 6, pp. 211-6, 1990 (PubMed).

Linsley, Clark, Ledbetter: "T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 13, pp. 5031-5, 1990 (PubMed).

Valle, Garrone, Yssel et al.: "mAb 104, a new monoclonal antibody, recognizes the B7 antigen that is expressed on activated B cells and HTLV-1-transformed T cells." in: Immunology, Vol. 69, Issue 4, pp. 531-5, 1990 (PubMed).

Freeman, Freedman, Segil et al.: "B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 8, pp. 2714-22, 1989 (PubMed).

Freedman, Freeman, Horowitz et al.: "B7, a B-cell-restricted antigen that identifies preactivated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 10, pp. 3260-7, 1987 (PubMed).