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CD80 antibody (CD80)

Details for Product anti-CD80 Antibody No. ABIN111250, Supplier: Log in to see
Antigen
  • CD8
  • Leu2
  • MAL
  • p32
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
Alternatives
anti-Human CD80 antibody for Immunoprecipitation
Reactivity
Human
299
221
49
15
10
9
8
3
2
2
Host
Mouse
272
109
99
59
16
13
Clonality (Clone)
Monoclonal ()
Conjugate
This CD80 antibody is un-conjugated
66
64
41
25
11
10
8
7
5
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
358
163
114
101
46
36
23
23
20
18
14
7
7
7
7
5
5
4
3
2
2
2
1
1
1
1
Supplier
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Immunogen Jijoye cells (Human Burkitt Lymphoma cell line)
Clone MAB104
Isotype IgG1
Specificity The antibody reacts with in vitro activated normal B cells. Reacts weakly with a small proportion of non-activated normal B cells. Does not react with peripheral monocytes, resting and activated normal T cells, T-cell lines and T-cell clones.
Purification Protein A affinity chromatography
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene ID 941
NCBI Accession NP_005182
UniProt P33681
Research Area Stem Cells, Hematopoietic Progenitors, Adaptive Immunity, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes Flow cytometry. Functional study of B cell subpopulations. Fluorescence microscopy or flow cytometry (2 g/5x10e5 cells/test). Immunohistochemistry (1: 20 to 1: 50, suitable on frozen sections or cell smears).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL of distilled water.
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer PBS containing 1.0 mg/mL BSA
Preservative Azide free
Storage 4 °C
Storage Comment Store at 2 - 8 °C.
Background publications Freedman, Freeman, Horowitz et al.: "B7, a B-cell-restricted antigen that identifies preactivated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 10, pp. 3260-7, 1987 (PubMed).

Freeman, Freedman, Segil et al.: "B7, a new member of the Ig superfamily with unique expression on activated and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 8, pp. 2714-22, 1989 (PubMed).

Vallé, Garrone, Yssel et al.: "mAb 104, a new monoclonal antibody, recognizes the B7 antigen that is expressed on activated B cells and HTLV-1-transformed T cells." in: Immunology, Vol. 69, Issue 4, pp. 531-5, 1990 (PubMed).

Linsley, Clark, Ledbetter: "T-cell antigen CD28 mediates adhesion with B cells by interacting with activation antigen B7/BB-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 13, pp. 5031-5, 1990 (PubMed).

June, Ledbetter, Linsley et al.: "Role of the CD28 receptor in T-cell activation." in: Immunology today, Vol. 11, Issue 6, pp. 211-6, 1990 (PubMed).

Vallé, Aubry, Durand et al.: "IL-4 and IL-2 upregulate the expression of antigen B7, the B cell counterstructure to T cell CD28: an amplification mechanism for T-B cell interactions." in: International immunology, Vol. 3, Issue 3, pp. 229-35, 1991 (PubMed).