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Cadherin 5 antibody (CDH5)

Details for Product anti-CDH5 Antibody No. ABIN111252, Supplier: Log in to see
Antigen
  • 7B4
  • AA408225
  • Cd144
  • VE-Cad
  • VECD
  • VEcad
  • Vec
  • wu:fc88a07
  • CD144
  • cadherin-5
Reactivity
Human
148
95
34
5
2
1
1
Host
Mouse
114
69
44
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Cadherin 5 antibody is un-conjugated
18
15
10
9
7
4
3
3
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
122
109
74
47
29
27
19
15
13
12
8
7
7
7
6
6
4
3
3
3
2
2
2
2
2
1
1
1
Supplier
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Immunogen Human endothelial cells (HUVEC)
Clone TEA1-31
Isotype IgG1
Alternative Name CD144 / Cadherin-5 (CDH5 Antibody Abstract)
Background Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha catenin forming a link to the cytoskeleton.Synonyms: 7B4 antigen, CDH5, VE-Cadherin, Vascular endothelial cadherin
Gene ID 9606
UniProt P33151
Research Area CD Antigens, Surface Receptors of Immune Cells, Extracellular Matrix, Signaling, Cytoskeleton
Pathways
Application Notes Suitable for use in Immunoprecipitation. Immunofluorescence: Working dilution on frozen sections 25-50 μg/mL. Incubation: 1 hour at 37C. Immunoelectroncytochemistry: Working dilution on ultra thin frozen sections: 25-50 μg/mL. Incubation overnight in a humid chamber at 2-8C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1 mL of distilled water
Buffer PBS with 1 mg/mL BSA, containing no preservative
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
Expiry Date 6 months
Background publications Fukudome, Yanagihara, Takeichi et al.: "Characterization of a mutant E-cadherin protein encoded by a mutant gene frequently seen in diffuse-type human gastric carcinoma." in: International journal of cancer. Journal international du cancer, Vol. 88, Issue 4, pp. 579-83, 2000 (PubMed).

Leach, Clark, Lampugnani et al.: "Immunoelectron characterisation of the inter-endothelial junctions of human term placenta." in: Journal of cell science, Vol. 104 ( Pt 4), pp. 1073-81, 1993 (PubMed).

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988 (PubMed).

Takeichi: "Cadherins: a molecular family important in selective cell-cell adhesion." in: Annual review of biochemistry, Vol. 59, pp. 237-52, 1990 (PubMed).

Takeichi: "Cadherin cell adhesion receptors as a morphogenetic regulator." in: Science (New York, N.Y.), Vol. 251, Issue 5000, pp. 1451-5, 1991 (PubMed).

Magee, Buxton: "Transmembrane molecular assemblies regulated by the greater cadherin family." in: Current opinion in cell biology, Vol. 3, Issue 5, pp. 854-61, 1991 (PubMed).