beta Endorphin antibody

Catalog No. ABIN111318

Product Details anti-beta Endorphin Antibody

Target: beta Endorphin (beta-EP) (Endorphin beta (beta-EP))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Endorphin antibody is un-conjugated

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein A affinity chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human beta-endorphin (full length native protein)

Clone: B31-15

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (paraffin sections): 10 - 20 mg/mL. Staining technique: Standard ABC technique (DAB+). Pretreatment: Heat retrieval (microwave oven). Incubation: overnight at 4 C. Positive tissue: Human pituitary gland. Immunohistochemistry (frozen sections).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH ~7.4, 15 mM Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-beta Endorphin antibody (ABIN111318)

Millenbaugh, Baskin, DeSilva, Elliott, Glickman: "Photothermal killing of Staphylococcus aureus using antibody-targeted gold nanoparticles." in: International journal of nanomedicine, Vol. 10, pp. 1953-60, (2016) (PubMed).

Fröbel, Cadeddu, Hartwig, Bruns, Wilk, Kündgen, Fischer, Schroeder, Steidl, Germing, Lehr, Haas, Czibere: "Platelet proteome analysis reveals integrin-dependent aggregation defects in patients with myelodysplastic syndromes." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 5, pp. 1272-80, (2013) (PubMed).

Meier, Köhler, Alkhatib, Sergi, Junker, Klein, Schmidt, Fritsch: "Beta-cell development and turnover during prenatal life in humans." in: European journal of endocrinology, Vol. 162, Issue 3, pp. 559-68, (2010) (PubMed).

Ubuka, Lai, Kitani, Suzuuchi, Pham, Cadigan, Wang, Chowdhury, Tsutsui, Bentley: "Gonadotropin-inhibitory hormone identification, cDNA cloning, and distribution in rhesus macaque brain." in: The Journal of comparative neurology, Vol. 517, Issue 6, pp. 841-55, (2009) (PubMed).

Stenholm, Hakanen, Vaarno, Pihlasalo, Terho, Hänninen, Vuopio-Varkila, Huovinen, Kotilainen: "Methicillin-resistant Staphylococcus aureus screening by online immunometric monitoring of bacterial growth under selective pressure." in: Antimicrobial agents and chemotherapy, Vol. 53, Issue 12, pp. 5088-94, (2009) (PubMed).

Hilgert, Stolba, Kristofová, Stefanová, Bendlová, Lebl, Horejsí: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, (1991) (PubMed).

Target Details for beta Endorphin

Target: beta Endorphin (beta-EP) (Endorphin beta (beta-EP))

Alternative Name: beta Endorphin (beta-EP Products)

Synonyms: ACTH antibody, BE antibody, Beta-LPH antibody, Clip antibody, Gamma-LPH antibody, Npp antibody, Pomc-1 antibody, Pomc1 antibody, alpha-MSH antibody, alphaMSH antibody, beta-MSH antibody, gamma-MSH antibody, CLIP antibody, LPH antibody, MSH antibody, NPP antibody, POC antibody, pro-opiomelanocortin-alpha antibody, proopiomelanocortin antibody, Pomc antibody, POMC antibody

Background: Beta-Endorphin is an endogenous opioid peptide neurotransmitter found in the neurons of both the central and peripheral nervous system. Beta-Endorphin is a peptide, 31 amino acids long, resulting from processing of the precursor pro-opiomelanocortin (POMC). Beta-Endorphin is found in neurons of the hypothalamus as well as the pituitary gland. It is an agonist of the opioid receptors, with evidence suggesting that it serves as the endogenous ligand of the μ-opioid receptor, the same receptor to which the chemicals extracted from opium, such as morphine and codeine, have their analgesic and addictive effects. Beta-Endorphin occurs in cells in the anterior pituitary. Beta-Endorphin may be detected in tumors of the pituitary, gastroinestinal and bronchial carcinoids. Absorption with 10-100 μg beta-Endorphin per ml diluted antiserum inactivates the antiserum, while Beta-lipotropin, met-enkephalin and leu-enkephalin do not.Synonyms: Endorfin-beta, Endorphin-beta

Pathways: Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity