anti-Apolipoprotein D antibody (APOD)

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Synonyms APOD, apod, zgc:153593, AOPDGN, Apo-D, lipocalin
(80), (33), (26), (1)
(62), (21), (9), (2)
Clonality (Clone)
Monoclonal ()
This Apolipoprotein D antibody is un-conjugated
(6), (6), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(71), (46), (25), (15), (12), (7), (6), (6), (1), (1)
Pubmed 7 references available
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Quantity 0.2 mL
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Immunogen Apo D, a progesterone binding glycoprotein of 24,000 dalton monomer molecular size.
Clone 8CD6
Isotype IgG1
Specificity Reacts with Human Apolipoprotein D.
Purification Ammonium Sulfate Precipitation and dialysis in PBS.
Alternative Name Apolipoprotein D (Apo D) (APOD Antibody Abstract)
Background Apo D is localized to steroid-responsive tissues (adrenal cortex, ovary, endometrium, prostate and pituitary). Apocrine epithelium within axillary skin exhibited strongly positive staining for Apo D and peripheral nerves, pituitary and renal tubules were also immunoreactive. Apo D, a Progesterone binding Glycoprotein of 24,000 dalton monomer molecular size, is a constituent of high density lipoprotein in plasma and was first isolated in large quantity as GCDFP-24, the major protein component of most human breast cyst fluids.Synonyms: APOD, Apo-D
Gene ID 347
NCBI Accession NP_001638
UniProt P05090
Research Area Metabolism
Application Notes Suitable for use in ELISA, Western blot and Immunohistochemistry on Paraffin (> 1/200).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Tissue Sections: Optimal staining: the sections should be pretreated with an antigen unmasking solution. The primary antibody should be incubated 20-60 minutes at room temperature. Dilutions: Primary antibody (concentrated format) may be diluted to > 1: 200 for Biotinbased detection systems.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Thomas, Dean, Pavey et al.: "Increased CNS levels of apolipoprotein D in schizophrenic and bipolar subjects: implications for the pathophysiology of psychiatric disorders." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 7, pp. 4066-71, 2001 (PubMed).

Kalman, McConathy, Araoz et al.: "Apolipoprotein D in the aging brain and in Alzheimer's dementia." in: Neurological research, Vol. 22, Issue 4, pp. 330-6, 2000 (PubMed).

Kolodny: "Niemann-Pick disease." in: Current opinion in hematology, Vol. 7, Issue 1, pp. 48-52, 2000 (PubMed).

Montpied, de Bock, Lerner-Natoli et al.: "Hippocampal alterations of apolipoprotein E and D mRNA levels in vivo and in vitro following kainate excitotoxicity." in: Epilepsy research, Vol. 35, Issue 2, pp. 135-46, 1999 (PubMed).

Terrisse, Poirier, Bertrand et al.: "Increased levels of apolipoprotein D in cerebrospinal fluid and hippocampus of Alzheimer's patients." in: Journal of neurochemistry, Vol. 71, Issue 4, pp. 1643-50, 1998 (PubMed).

Ong, He, Suresh et al.: "Differential expression of apolipoprotein D and apolipoprotein E in the kainic acid-lesioned rat hippocampus." in: Neuroscience, Vol. 79, Issue 2, pp. 359-67, 1997 (PubMed).

Aspinall, Bentel, Horsfall et al.: "Differential expression of apolipoprotein-D and prostate specific antigen in benign and malignant prostate tissues." in: The Journal of urology, Vol. 154, Issue 2 Pt 1, pp. 622-8, 1995 (PubMed).

Catalog No. ABIN111355
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