anti-CD3 antibody (CD3)

Details for Product anti-CD3 Antibody No. ABIN111380
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(610), (189), (69), (40), (35), (29), (23), (16), (13), (12), (10), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6)
(649), (122), (86), (52), (1)
Clonality (Clone)
Monoclonal ()
This CD3 antibody is un-conjugated
(154), (92), (82), (42), (11), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Flow Cytometry (FACS)
(693), (172), (129), (120), (111), (95), (69), (67), (56), (27), (11), (10), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Pubmed 4 references available
Quantity 0.2 mg
Shipping to United States ( )
Availability Discontinued
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Immunogen Human peripheral blood Lymphocytes.
Clone X35
Isotype IgG2a
Specificity The molecular weights of the recognized antigens are 26, 28, 20, 20, and 16 kDa for the gamma, eta, delta, epsilon, and zeta chains respectively. This product recognizes the CD3 antigen expressed on the mature T Lymphocytes. T Lymphocytes receive the antigenic information through the T3/Ti complex. The CD3 antigen has 5 glycoprotein chains non-covalently associated with the T-cell receptor. Activation of T Lymphocytes can be induced when foreign antigen is fixed to the T cell receptor. The antibody also has a mitogenic effect on T lymphocytes. Does not react with those T cells, defined by E. Rosette technique, lacking the T cell receptor.
Alternative Name CD3 (CD3 Antibody Abstract)
Background T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Alternate names: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4
Gene ID 916
NCBI Accession NP_000724
UniProt P07766
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
Application Notes Study of T cell functions. Fluorescence microscopy or Flow Cytometry: 2 µg/5x10e5 cells/test. Immunohistochemistry: Working dilution 1/25-1/50. This antibody is only suitable on Frozen Sections or cell smears. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with 1 mL of distilled water.
Concentration 0.2 mg/mL (prior to lyophilization)
Buffer PBS containing 1 mg/ml BSA without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Stable for one year after reconstitution when stored at 2-8°C. For extended storage keep in aliquots at -20°C.
Expiry Date 12 months
Background publications Thorner: "An essential role for cyclic AMP in growth control: the case for yeast." in: Cell, Vol. 30, Issue 1, pp. 5-6, 1982 (PubMed).

Spits, Yssel, Leeuwenberg et al.: "Antigen-specific cytotoxic T cell and antigen-specific proliferating T cell clones can be induced to cytolytic activity by monoclonal antibodies against T3." in: European journal of immunology, Vol. 15, Issue 1, pp. 88-91, 1985 (PubMed).

General Reinherz, Meuer, Fitzgerald et al.: "Antigen recognition by human T lymphocytes is linked to surface expression of the T3 molecular complex." in: Cell, Vol. 30, Issue 3, pp. 735-43, 1983 (PubMed).

Lum: "The kinetics of immune reconstitution after human marrow transplantation." in: Blood, Vol. 69, Issue 2, pp. 369-80, 1987 (PubMed).

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Catalog No. ABIN111380
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