CD3 (CD3E) antibody
| Antigen |
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| Synonyms |
T3E, TCRE, FLJ18683 |
| Clonality |
Monoclonal (X35) |
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Host
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Reactivity
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Conjugate
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FITC (74), Biotin (45), PE (37), APC (26), RPE (7), Cy5 (5), PerCP (5), Cy5,PE (4), PE-Dyomics 647 (4), Alexa Fluor 488 (3), RPE,Cy5 (3), SPRD (3), APC,Cy7 (2), Cy7,PE (2), Dyomics 647 (2), PE,Cy7 (2), PE-Cy5 (2), PE-Dyomics 590 (2), PerCP-Cy5.5 (2), Alexa Fluor 647 (1), CF405M (1), Cy5.5 (1), Orange Cytognos 515 (1), PE,Cy5.5 (1), PE,Dyomics 647 (1), RPE,Cy7 (1)
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Application
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 Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Functional Studies (Func), Flow Cytometry (FACS)
Flow Cytometry (FACS) (323), Immunoprecipitation (IP) (82), Functional Studies (Func) (71), Immunohistochemistry (Frozen Sections) (IHC (fro)) (64), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (46), Immunohistochemistry (Fixed) (IHC (fx)) (33), ELISA (24), Immunofluorescence (IF) (23), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunostaining (ISt) (4), Activation (Act) (3), Blocking Antibody (Inhibition) (3), Blocking Antibody (Inhibition) (2), Cytotoxicity Test (CyTox) (1), Dot Blot (Dot) (1)
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| Catalog no. |
ABIN111380 |
| Quantity |
0.2 mg (0.2 mg/ml (prior to lyophilization)) |
| Price |
484.00 $ Plus shipping costs $45.00
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| Shipping to |
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| Availability |
Will be delivered in 6 to 8 Business Days |
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Alternative name
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CD3
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UniProt
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P07766
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Immunogen
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Human peripheral blood Lymphocytes.
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Cross-Reactivity
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Human
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Isotype
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IgG2a
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Clone
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X35
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Description
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T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of theCD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits arestructurally related members of the immunoglobulins super family encoded by closelylinked genes on human chromosome 11. The CD3 components have long cytoplasmic tailsthat associate with cytoplasmic signal transduction molecules. This association ismediated at least in part by a double tyrosine based motif present in a single copy in theCD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival andproliferation. The CD3 antigen is present on 68-82% of normal peripheral bloodlymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is neverexpressed on B or NK cells. Decreased percentages of T lymphocytes may be observed insome autoimmune diseases.
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Characteristics
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Synonyms: T3/Leu-4, T-cell surface glycoprotein CD3, T-cell surface antigen T3/Leu-4
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Application Notes
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Study of T cell functions. Fluorescence microscopy or Flow Cytometry: 2 µg/5x10e5 cells/test. Immunohistochemistry: Working dilution 1/25-1/50. This antibody is only suitable on Frozen Sections or cell smears. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Concentration
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0.2 mg/ml (prior to lyophilization)
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Buffer
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PBS containing 1 mg/ml BSA without preservatives.Reconstitution: Restore with 1 ml of distilled water.
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Preservative
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Azide Free
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Storage
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Store lyophilized (preferably in a desiccator) at 2-8°C and reconstituted (in aliquots) at-20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. The addition of 0. 09% (w/v) sodium azide is recommended for storage of the reconstitutedform for up to one month at 2-8°C.
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Research Area
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Immunology, Adaptive Immunity, Hematopoietic Progenitors, Hematopoietic Stem Cells, CD Antigens, Surface Receptors of Immune Cells
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Restrictions
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For Research Use only
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Lum: "The kinetics of immune reconstitution after human marrow transplantation." in: Blood, Vol. 69, Issue 2, pp. 369-80, 1987 (PubMed).
Reinherz, Meuer, Fitzgerald et al.: "Antigen recognition by human T lymphocytes is linked to surface expression of the T3 molecular complex." in: Cell, Vol. 30, Issue 3, pp. 735-43, 1983 (PubMed).
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Alternatives for antigen "CD3 (CD3E)", type "Antibodies"
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Hosts
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Mouse (313), Rat (52), Rabbit (47), Hamster (38), Armenian Hamster (1)
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Reactivities
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Human (324), Mouse (Murine) (99), Rat (Rattus) (26), Primate (21), Dog (Canine) (9), Monkey (3), Rhesus Monkey (1)
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Applications
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Flow Cytometry (FACS) (323), Immunoprecipitation (IP) (82), Functional Studies (Func) (71), Immunohistochemistry (Frozen Sections) (IHC (fro)) (64), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (53), Western Blotting (WB) (46), Immunohistochemistry (Fixed) (IHC (fx)) (33), ELISA (24), Immunofluorescence (IF) (23), Immunohistochemistry (IHC) (15), Immunocytochemistry (ICC) (14), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (4), Immunostaining (ISt) (4), Activation (Act) (3), Blocking Antibody (Inhibition) (3), Blocking Antibody (Inhibition) (2), Cytotoxicity Test (CyTox) (1), Dot Blot (Dot) (1)
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Conjugates
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FITC (74), Biotin (45), PE (37), APC (26), RPE (7), Cy5 (5), PerCP (5), Cy5,PE (4), PE-Dyomics 647 (4), Alexa Fluor 488 (3), RPE,Cy5 (3), SPRD (3), APC,Cy7 (2), Cy7,PE (2), Dyomics 647 (2), PE,Cy7 (2), PE-Cy5 (2), PE-Dyomics 590 (2), PerCP-Cy5.5 (2), Alexa Fluor 647 (1), CF405M (1), Cy5.5 (1), Orange Cytognos 515 (1), PE,Cy5.5 (1), PE,Dyomics 647 (1), RPE,Cy7 (1)
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Epitopes
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AA 156-168 (6), Chain epsilon (6), Activation Site (4), AA 156-168,AA 156-16 (1), AA 196-206 (1), C-Term (1)
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