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Details for Product No. ABIN111459

Spectrin Binding Protein antibody

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Antigen
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Application
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN111459
Quantity 0.2 mL
Price
1,270.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant Hssh3bp1 (human spectrin SH3 domain binding protein 1)
Clone 4E2
Isotype IgG2b
Specificity This antibody was developed against the spectrin SH3 domain binding protein Hssh3bp1 (3).
Purification Protein A Chromatography
Background Hssh3bp1 is a marker of macropinocytic vesicles and a potential regulator of macropinocytosis (2). The gene encoding Hssh3bp1 has a role as a tumor suppressor gene which is inactivated during prostate tumorigenesis (1).
Alternate names: ABI1, Abelson interactor 1, Abi-1, Abl interactor 1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Nap1-binding protein, SSH3BP1, Spectrin SH3 domain-binding protein 1, e3B1
Gene ID 10006
NCBI Accession NP_001012768
UniProt Q8IZP0
Application Notes Western blot: 1/1,000-1/2,000. Immunofluorescence: 1/100-1/500. Immunohistochemistry: 1/40-1/100. Formalin-fixed, paraffin-embedded sections andfrozen sections. For optimal staining, sections should be pre-treated for antigenunmasking. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS without preservatives
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Expiry Date 12 months
Background publications Lamaze, Schmid: "The emergence of clathrin-independent pinocytic pathways." in: Current opinion in cell biology, Vol. 7, Issue 4, pp. 573-80, 1996 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Ziemnicka-Kotula, Xu, Gu et al.: "Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrin-based membrane skeleton." in: The Journal of biological chemistry, Vol. 273, Issue 22, pp. 13681-92, 1998 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Macoska, Xu, Ziemnicka et al.: "Loss of expression of human spectrin src homology domain binding protein 1 is associated with 10p loss in human prostatic adenocarcinoma." in: Neoplasia (New York, N.Y.), Vol. 3, Issue 2, pp. 99-104, 2001 (PubMed).

Swanson, Watts: "Macropinocytosis." in: Trends in cell biology, Vol. 5, Issue 11, pp. 424-8, 2004 (PubMed).

Alternatives for antigen "Spectrin Binding Protein", type "Antibodies"
Hosts (2)
Reactivities (1)
Applications (2), (2), (1), (1), (1)
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