Spectrin Binding Protein antibody

Antigen: Spectrin Binding Protein

Clonality: Monoclonal (4E02)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN111459

Additional Information

Gene ID: 10006

UniProt: Q8IZP0

Immunogen: Recombinant Hssh3bp1 (human spectrin SH3 domain binding protein 1)

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG2b

Clone: 4E02

Description: Hssh3bp1 is a marker of macropinocytic vesicles and a potential regulator ofmacropinocytosis (2). The gene encoding Hssh3bp1 has a role as a tumor suppressor genewhich is inactivated during prostate tumorigenesis (1).

Characteristics: Synonyms: ABI1, SSH3BP1, Abl interactor 1, Abelson interactor 1, Abi-1, e3B1, Nap1-binding protein,Abl-binding protein 4, AblBP4, Spectrin SH3 domain-binding protein 1, Eps8 SH3domain-binding protein

Specificity: This antibody was developed against the spectrin SH3 domain binding protein Hssh3bp1(3). Species: Human, Rat, Mouse. Others not tested.

Application Details

Application Notes: Western blot: 1/1,000-1/2,000. Immunofluorescence: 1/100-1/500. Immunohistochemistry: 1/40-1/100. Formalin-fixed, paraffin-embedded sections andfrozen sections. For optimal staining, sections should be pre-treated for antigenunmasking. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS without preservatives

Storage: Store the antibody at -20°C. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Lamaze, Schmid: "The emergence of clathrin-independent pinocytic pathways." in: Current opinion in cell biology, Vol. 7, Issue 4, pp. 573-80, 1996 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Ziemnicka-Kotula, Xu, Gu et al.: "Identification of a candidate human spectrin Src homology 3 domain-binding protein suggests a general mechanism of association of tyrosine kinases with the spectrin-based membrane skeleton." in: The Journal of biological chemistry, Vol. 273, Issue 22, pp. 13681-92, 1998 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Macoska, Xu, Ziemnicka et al.: "Loss of expression of human spectrin src homology domain binding protein 1 is associated with 10p loss in human prostatic adenocarcinoma." in: Neoplasia (New York, N.Y.), Vol. 3, Issue 2, pp. 99-104, 2001 (PubMed).

Swanson, Watts: "Macropinocytosis." in: Trends in cell biology, Vol. 5, Issue 11, pp. 424-8, 2004 (PubMed).