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Spectrin alpha 1, Erythrocytic (SPTA1) (SH3 Domain) antibody

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SH3 Domain
(1), (1)
Human, Mouse (Murine), Rat (Rattus)
(12), (3), (3), (1)
(7), (6)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(11), (5), (4), (4), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
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Quantity 0.2 mL
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Immunogen Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.
Clone 17C7
Isotype IgG1
Specificity This antibody was developed against the alpha I spectrin SH3 domain (2). Specific marker for human, rat and mouse alpha I spectrin. Reacts with erythroid spectrin in red blood cells and in cerebellum. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinant human fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization of erythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 200 kDa protein which is a candidate marker of endocytic vesicles called macropinosomes (1,2,5).
Purification Ammonium Sulfate Precipitation and dialysis.
Alternative Name Spectrin alpha Chain (Erythrocyte) / SPTA1 (SPTA1 Antibody Abstract)
Background Synonyms: Erythroid alpha-spectrin, SPTA
Gene ID 6708
NCBI Accession NP_003117
UniProt P02549
Application Notes Suitable for use in ELISA (1/10e5-1/10^6), Western blot (1/10e2-1/10e3),Immunofluorescence (1/100-1/500) and Immunohistochemistry (1/10-1/50, Frozen only.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, containing 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and in aliquots at -80 °C. Reconstituted antibody can be stored at 4 °C for a limited period of time, it does not show decline in activity after two weeks at 4 °C.
Background publications Swanson, Watts: "Macropinocytosis." in: Trends in cell biology, Vol. 5, Issue 11, pp. 424-8, 2004 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Lamaze, Schmid: "The emergence of clathrin-independent pinocytic pathways." in: Current opinion in cell biology, Vol. 7, Issue 4, pp. 573-80, 1996 (PubMed).

Catalog No. ABIN111460

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