Spectrin alpha chain (erythrocyte) / SPTA1 (alpha 1) (SH3 Domain) antibody

Antigen: Spectrin alpha chain (erythrocyte) / SPTA1 (alpha 1)

Binding Site: SH3 Domain

Clonality: Monoclonal (17C7)

Host: Mouse

Reactivity: Human

Application: Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN111460

Additional Information

Gene ID: 6708

UniProt: P02549

Immunogen: Recombinant alpha I spectrin (erythroid spectrin) SH3 domain.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Isotype: IgG1

Clone: 17C7

Characteristics: Synonyms: SPTA, Erythroid alpha-spectrin

Specificity: This antibody was developed against the alpha I spectrin SH3 domain (2). Specific marker for human, rat and mouse alpha I spectrin. Reacts with erythroid spectrinin red blood cells and in cerebellum. Does not cross react with alpha II (non-erythroid) spectrin SH3 domain or recombinanthuman fodrin SH3 domain. In cerebellar cell cultures, clone 17C7 labels cell bodies consistent with localization oferythroid spectrin. In NIH 3T3 cells, clone 17C7 reacts with a 200 kDa protein which is acandidate marker of endocytic vesicles called macropinosomes (1,2,5). Species: Human. Others not tested.

Application Details

Application Notes: Suitable for use in ELISA (1/10e5-1/10e6), Western blot (1/10e2-1/10e3),Immunofluorescence (1/100-1/500) and Immunohistochemistry (1/10-1/50, Frozen only. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Purified

Buffer: PBS, containing 0.09% Sodium Azide as preservative.

Storage: Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only

Publications

Lamaze, Schmid: "The emergence of clathrin-independent pinocytic pathways." in: Current opinion in cell biology, Vol. 7, Issue 4, pp. 573-80, 1996 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).

Swanson, Watts: "Macropinocytosis." in: Trends in cell biology, Vol. 5, Issue 11, pp. 424-8, 2004 (PubMed).