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Details for Product No. ABIN111461

Spectrin alpha Chain, Brain (SPTAN1) (SH3 Domain) antibody

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Synonyms EIEE5, NEAS, SPTA2, 2610027H02Rik, Spna-2, Spna2, A2a, IPF, SPECA, im:7157190, wu:fa20e05, wu:fb33g08, wu:fk32a10, zgc:112229
»Alternatives SH3 Domain
»Alternatives Human, Mouse (Murine), Rat (Rattus)
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
»Alternatives Un-conjugated
»Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 7 references available
Catalog no. ABIN111461
Quantity 0.2 mL
1,270.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain.
Clone D8B7
Isotype IgG2b
Specificity This antibody was developed against the alpha II spectrin SH3 domain (1). Specific marker for human, rat and mouse alpha II spectrin or fodrin. In cerebellar cell cultures, it labels axons and periphery of cell bodies consistent with localization of fodrin (3, 5, 7). Does not cross-react with alpha I (erythroid) spectrin SH3 domain.
Purification Ammonium sulfate precipitation and dialysis
Alternative Name Spectrin alpha chain (brain) / SPTAN1
Background Fodrin is a universally expressed membrane associated cytoskeletal protein consisting of alpha and beta subunits. The protein is important for maintaining normal membrane structure and supporting cell surface protein function. Alpha Fodrin is one of the important targets cleaved by caspases during apoptosis. The full length 240 kDa protein can be cleaved at several sites within its sequence by activated caspases generating N terminal 150 kDa, C terminal 120 kDa, and 35 kDa major products. Cleavage of alpha Fodrin leads to membrane malfunction and cell shrinkage.
Alternate names: Alpha-II spectrin, Fodrin alpha chain, SPTA2, Spectrin non-erythroid alpha chain
Gene ID 6709
NCBI Accession NP_001123910.1
UniProt Q13813
Application Notes ELISA: 1/100,000-1/1,000,000. Western blot: 1/100-1/1,000. Immunofluorescence: 1/100-1/500. Immunohistochemistry on frozen sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.09% Sodium Azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Lamaze, Schmid: "The emergence of clathrin-independent pinocytic pathways." in: Current opinion in cell biology, Vol. 7, Issue 4, pp. 573-80, 1996 (PubMed).

Goodman, Zimmer, Clark et al.: "Brain spectrin: of mice and men." in: Brain research bulletin, Vol. 36, Issue 6, pp. 593-606, 1995 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

De Matteis, Morrow: "Spectrin tethers and mesh in the biosynthetic pathway." in: Journal of cell science, Vol. 113 ( Pt 13), pp. 2331-43, 2000 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).

Swanson, Watts: "Macropinocytosis." in: Trends in cell biology, Vol. 5, Issue 11, pp. 424-8, 2004 (PubMed).

Alternatives for antigen "Spectrin alpha Chain, Brain (SPTAN1)", type "Antibodies"
Hosts (53), (13)
Reactivities (51), (22), (21), (15), (13), (7), (1), (1), (1), (1), (1), (1)
Applications (55), (29), (13), (13), (13), (12), (10), (6), (6), (4), (2), (2), (1), (1), (1)
Conjugates (7), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (5), (4), (2), (2), (2), (2), (1), (1), (1), (1), (1)