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Spectrin alpha Chain, Brain (SPTAN1) (SH3 Domain) antibody

Antigen

Spectrin alpha Chain, Brain (SPTAN1)
Synonyms NEAS, FLJ44613, (ALPHA)II-SPECTRIN, MGC112229, im:7157190, wu:fa20e05, wu:fb33g08, wu:fk32a10, zgc:112229
Binding Site
Alternatives

SH3 Domain
Clonality Monoclonal (D8B7)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
6 references available
Catalog no. ABIN111461
Quantity 0.2 mL  (1.0 mg/ml)
Price 968.00 $   Plus shipping costs $45.00
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Additional Information
Application Details Publications Alternatives back to top

Alternative name Spectrin alpha chain (brain) / SPTAN1
UniProt Q13813
Immunogen Recombinant alpha II spectrin (non-erythroid spectrin or fodrin) SH3 domain.
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Isotype IgG2b
Clone D8B7
Description Fodrin is a universally expressed membrane associated cytoskeletal protein consisting ofalpha and beta subunits. The protein is important for maintaining normal membranestructure and supporting cell surface protein function. Alpha Fodrin is one of the importanttargets cleaved by caspases during apoptosis. The full length 240 kDa protein can becleaved at several sites within its sequence by activated caspases generating N terminal150 kDa, C terminal 120 kDa, and 35 kDa major products. Cleavage of alpha Fodrin leads tomembrane malfunction and cell shrinkage.
Characteristics Synonyms: Spectrin non-erythroid alpha chain, Alpha-II spectrin, Fodrin alpha chain, SPTA2

Application Details
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Application Notes ELISA: 1/100,000-1/1,000,000. Western blot: 1/100-1/1,000. Immunofluorescence: 1/100-1/500. Immunohistochemistry on frozen sections: 1/50-1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Concentration 1.0 mg/ml
Purification Purified
Buffer PBS containing 0.09% Sodium Azide as preservative
Storage Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Should this product contain a precipitate we recommend microcentrifugation before use. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Lamaze, Schmid: "The emergence of clathrin-independent pinocytic pathways." in: Current opinion in cell biology, Vol. 7, Issue 4, pp. 573-80, 1996 (PubMed).

Goodman, Zimmer, Clark et al.: "Brain spectrin: of mice and men." in: Brain research bulletin, Vol. 36, Issue 6, pp. 593-606, 1995 (PubMed).

Bennett, Gilligan: "The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane." in: Annual review of cell biology, Vol. 9, pp. 27-66, 1994 (PubMed).

Xu, Ziemnicka, Merz et al.: "Human spectrin Src homology 3 domain binding protein 1 regulates macropinocytosis in NIH 3T3 cells." in: Journal of cell science, Vol. 113 Pt 21, pp. 3805-14, 2000 (PubMed).

Xu, Ziemnicka, Scalia et al.: "Monoclonal antibodies to alphaI spectrin Src homology 3 domain associate with macropinocytic vesicles in nonerythroid cells." in: Brain research, Vol. 898, Issue 1, pp. 171-7, 2001 (PubMed).

Swanson, Watts: "Macropinocytosis." in: Trends in cell biology, Vol. 5, Issue 11, pp. 424-8, 2004 (PubMed).