C-Peptide antibody

Antigen: C-Peptide

Clonality: Monoclonal (C-PEP-01)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111474

Additional Information

Immunogen: C-Peptide of Human Proinsulin

Format: Ascites

Isotype: IgG1  (Matching secondary antibodies)

Clone: C-PEP-01

Description: A precursor of Insulin it is converted to Insulin by removal of the connecting C peptide,leaving the two (A and B) chains. From every molecule of Proinsulin, one molecule ofInsulin plus one molecule of C Peptide are produced. C Peptide is part of the molecule of Proinsulin, that consists of three parts: C Peptide andtwo long strands of amino acids (called the alpha and beta chains) that later becomelinked together to form the insulin molecule. From every molecule of proinsulin, onemolecule of insulin plus one molecule of C Peptide are produced. C peptide is releasedinto the blood stream in equal amounts to insulin. A test of C peptide levels will show howmuch insulin the body is making. Insulin decreases blood glucose concentration. Itincreases cell permeability to monosaccharides, amino acids and fatty acids. It acceleratesglycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Specificity: This anitbody reacts with the C-peptide of Human Proinsulin. In normal tissues it reacts with normal pancreatic islet Beta-cells. In tumor tissues it stains Insulin secreting neoplasms (insulinomas). The antibody reacts with Human Proinsulin but not with Insulin, Glucagon or Somatostatin,and recognizes the amino acid residues 8-13 and 25-31. Species Reactivity: Tested: Human.

Application Details

Protocol: Protocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Application Notes: Immunohistochemistry on Paraffin Sections: 0.2 µg/ml (1/5000) (No pretreatmentnecessary). Recommended Positive Control: Human pancreas. Has been described to work in ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Buffer: PBS, pH 7.2 with 10 mg/ml BSA as a stabilizer and 0.09% Sodium Azide asa preservative.Reconstitution: Restore with 0.5 ml distilled water.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Do Not Freeze working dilutionsAvoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Diabetes, Hormones

Restrictions: For Research Use only

Publications

Publications: Hilgert, Stolba, Kristofova et al.: "A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA." in: Hybridoma, Vol. 10, Issue 3, pp. 379-86, 1991 (PubMed).