Selectin E/CD62e antibody (FITC)

Catalog No. ABIN111607

Product Details anti-Selectin E/CD62e Antibody (FITC)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody recognizes the CD62E and CD62P cell surface antigens. Although previously thought to recognise only Human CD62E, recent data shows that this antibody also recognizes Human CD62P, binding to a common epitope shared by these members of the selectin family.

Cross-Reactivity (Details): Species reactivity (expected):Pig.
Species reactivity (tested):Human.
Also reacts to: CD62P

Purification: Affinity Chromatography on Protein A.

Immunogen: Human E-Selectin (ELAM-1). Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.

Clone: 1-2B6

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: use 10 μL of neat antibody to label 1 x 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: CD62E / E-Selectin (SELE Products)

Synonyms: SELE antibody, CD62E antibody, ELAM antibody, ELAM1 antibody, ESEL antibody, LECAM2 antibody, E-selectin antibody, Elam antibody, selectin E antibody, selectin, endothelial cell antibody, SELE antibody, Sele antibody

Background: CD62E is also known as endothelial leukocyte adhesion molecule 1 (ELAM1) or E selectin. It is a type I membrane protein that belongs to the selectin family of surface molecules (along with CD62L and CD62P). Selectins are C type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. CD62E (E selectin) is an endothelial cell specific selectin that is expressed on cytokine induced endothelial cells only after activation with proinflammatory cytokines. In vitro experiments have shown that IL1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of CD62E in an NFkB dependent signalling cascade. CD62E has been associated with blood vessel endothelium in diverse inflammatory situations. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x.Synonyms: CD62 antigen-like family member E, ELAM1, Endothelial leukocyte adhesion molecule 1, LECAM2, Leukocyte-endothelial cell adhesion molecule 2, SELE

Gene ID: 6401

NCBI Accession: NP_000441

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling