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Desmoglein 2 antibody (DSG2)

Details for Product anti-DSG2 Antibody No. ABIN111817, Supplier: Log in to see
Antigen
  • AA408168
  • ARVC10
  • ARVD10
  • CDHF5
  • CMD1BB
  • D18Ertd293e
  • dsc
  • DSG2
  • dsga
  • HDGC
  • si:dkeyp-51f12.6
  • wu:fj23f02
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
109
26
26
Host
Mouse
78
32
Clonality (Clone)
Monoclonal ()
Conjugate
This Desmoglein 2 antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
92
38
30
20
19
16
13
11
6
6
5
2
1
1
1
Supplier
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Immunogen "Band 3" polypeptide of isolated desmosomes from Bovine muzzle epidermis
Clone DG3-10
Isotype IgG1
Specificity This antibody recognizes Desmoglein 1+2 (desmosome-specific cadherins) in desmosome-bearing cells. It does not react with cells containing plaque-bearing junctions of non-desmosomal type. Tumors Specifically Detected: All epithelium-derived tumors, notably carcinomas, and meningiomas. Polypeptide Reacting: Mr 165000 Desmoglein, "band 3" polypeptide of the desmosomal complex. Epitope Recognized: Localized at the repeating unit domain ("RUD") of the cytoplasmic C-terminal part of the polypeptide. Reactivities on Cultured Cell Lines (tested so far): A-431, MCF-7, TR-146, PLC and various other Human epithelial cell lines, Bovine BMGE+H, BMGE-H, MDBK.
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Rat and Mouse.
Purification Affinity Chromatography on Protein A
Alternative Name Desmoglein-2 (DSG2 Antibody Abstract)
Background Desmosomes are cell-cell junctions between epithelial, myocardial and certain other cell types. Desmoglein 1 and 2 are calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Currently, three desmoglein subfamily members have been identified and all are members of the cadherin cell adhesion molecule superfamily. These desmoglein gene family members are located in a cluster on chromosome 18.Synonyms: CDHF4, CDHF5, DG1, DGI, DSG-1, DSG-2, DSG1, DSG2, Desmoglein1, Desmoglein2, Desmosomal glycoprotein 1, HDGC, Pemphigus foliaceus antigen
Gene ID 1829
NCBI Accession NP_001934
UniProt Q14126
Research Area Cytoskeleton
Application Notes Western blotting. Immunofluorescence Microscopy. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Embedded Tissue (after microwave treatment). Working Dilution: Dilute at least 1/10 with PBS, pH 7.4, for Immunohistochemicalapplication. Incubation time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 1.0 mL distilled water.
Buffer Final Solution contains PBS, pH 7.4 with 0.09 % Sodium Azide as preservative and 0.5 % BSA as stabilizer
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Ponte-Sucre, Moll: "Parasites, flies and men--21st Meeting of the German Society of Parasitology in Würzburg." in: European journal of cell biology, Vol. 84, Issue 1, pp. 7-13, 2005

Bosch, Andl, Abel, Kartenbeck: "E-cadherin is a selective and strongly dominant prognostic factor in squamous cell carcinoma: a comparison of E-cadherin with desmosomal components." in: International journal of cancer. Journal international du cancer, Vol. 114, Issue 5, pp. 779-90, 2005

Akat, Mennel, Kremer, Gassler, Bleck, Kartenbeck: "Molecular characterization of desmosomes in meningiomas and arachnoidal tissue." in: Acta neuropathologica, Vol. 106, Issue 4, pp. 337-47, 2003

Caldelari, de Bruin, Baumann, Suter, Bierkamp, Balmer, Müller: "A central role for the armadillo protein plakoglobin in the autoimmune disease pemphigus vulgaris." in: The Journal of cell biology, Vol. 153, Issue 4, pp. 823-34, 2001

Hatzfeld, Haffner, Schulze, Vinzens: "The function of plakophilin 1 in desmosome assembly and actin filament organization." in: The Journal of cell biology, Vol. 149, Issue 1, pp. 209-22, 2000

Schäfer, Stumpp, Franke: "Immunological identification and characterization of the desmosomal cadherin Dsg2 in coupled and uncoupled epithelial cells and in human tissues." in: Differentiation; research in biological diversity, Vol. 60, Issue 2, pp. 99-108, 1996

Yang, Shaio, Wang, Wu, Stone: "Neuroblastoma cell-mediated leukocyte chemotaxis: lineage-specific differentiation of interleukin-8 expression." in: Experimental cell research, Vol. 211, Issue 1, pp. 1-5, 1994

Schmelz, Franke: "Complexus adhaerentes, a new group of desmoplakin-containing junctions in endothelial cells: the syndesmos connecting retothelial cells of lymph nodes." in: European journal of cell biology, Vol. 61, Issue 2, pp. 274-89, 1993

Koch, Walsh, Schmelz, Goldschmidt, Zimbelmann, Franke: "Identification of desmoglein, a constitutive desmosomal glycoprotein, as a member of the cadherin family of cell adhesion molecules." in: European journal of cell biology, Vol. 53, Issue 1, pp. 1-12, 1991