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CD68 antibody (Biotin)

CD68 Reactivity: Rat FACS Host: Mouse Monoclonal ED1 Biotin
Catalog No. ABIN111831
  • Target See all CD68 Antibodies
    CD68 (CD68 Molecule (CD68))
    Reactivity
    • 156
    • 76
    • 44
    • 39
    • 26
    • 14
    • 10
    • 2
    • 2
    • 2
    • 2
    Rat
    Host
    • 89
    • 86
    • 11
    • 1
    Mouse
    Clonality
    • 102
    • 81
    Monoclonal
    Conjugate
    • 86
    • 16
    • 14
    • 9
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CD68 antibody is conjugated to Biotin
    Application
    • 76
    • 76
    • 50
    • 37
    • 36
    • 34
    • 33
    • 24
    • 14
    • 13
    • 12
    • 10
    • 8
    • 4
    • 3
    • 2
    • 1
    Flow Cytometry (FACS)
    Specificity
    CD68 antibody (Cat#BM4000B) recognises a single chain glycoprotein of 110 kDa that is expressed predominantly on the lysomal membrane of myeloid cells. Weak cell surface expression also occurs. The antigen is expressed by the majority of tissue macrophages, and weakly be peripheral blood granulocytes. Studies have shown that the antigen recognised by ED1 is the rat homologue of human CD68.
    Purification
    Affinity Chromatography on Protein A
    Immunogen
    Rat spleen cells
    Clone
    ED1
    Isotype
    IgG1
    Top Product
    Discover our top product CD68 Primary Antibody
  • Application Notes
    Flow Cytometry: Use 10 mLof neat antibody to label 10^6 cells in 100 μL. (Results maybe enhanced using membrane permeabilisation).
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    0.1 mg/mL
    Buffer
    PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 1 % BSA as stabiliser
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Schneider, Meusel, Renker, Bauer, Holzinger, Roeder, Wanner, Gekle, Sauvant: "Low-dose indomethacin after ischemic acute kidney injury prevents downregulation of Oat1/3 and improves renal outcome." in: American journal of physiology. Renal physiology, Vol. 297, Issue 6, pp. F1614-21, (2009) (PubMed).

  • Target
    CD68 (CD68 Molecule (CD68))
    Alternative Name
    CD68 (CD68 Products)
    Synonyms
    Lamp4 antibody, Scard1 antibody, gp110 antibody, GP110 antibody, LAMP4 antibody, SCARD1 antibody, CD68 molecule antibody, CD68 antigen antibody, Cd68 molecule antibody, CD68 antibody, Cd68 antibody
    Background
    The CD68 antigen is a 37kD transmembrane protein that is post-translationally glycosylated to give a protein of 87-115kD. CD68 is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. It could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin bearing substrates or other cells.Synonyms: Gp110, Macrophage marker, Macrosialin
    Gene ID
    287435
    NCBI Accession
    NP_001026808
    UniProt
    Q4FZY1
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