Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

SFTPD antibody (Lectin Domain)

SFTPD Reactivity: Human, Rat WB, IHC (p), IHC (fro), EIA Host: Mouse Monoclonal VIF11 unconjugated
Catalog No. ABIN111841
  • Target See all SFTPD Antibodies
    SFTPD (Surfactant Protein D (SFTPD))
    Binding Specificity
    • 8
    • 8
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Lectin Domain
    Reactivity
    • 52
    • 21
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 52
    • 17
    Mouse
    Clonality
    • 52
    • 17
    Monoclonal
    Conjugate
    • 33
    • 15
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SFTPD antibody is un-conjugated
    Application
    • 54
    • 32
    • 20
    • 10
    • 8
    • 8
    • 5
    • 5
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    Clone VI F11 is useful for staining rat and human surfactant protein D . Antigen Distribution on Tissues and Cells: Alveolar type II cells, alveolar macrophages, two types of Clara cells SP-A+ and SP-A-, and extracellular deposits. Alveolar type III cells stain negative. No reaction on Rat skin, colon, kidney and liver.
    Cross-Reactivity (Details)
    Species reactivity (tested):Rat and Human. Negative with Pig
    Purification
    Affinity Chromatography
    Immunogen
    Purified Rat Surfactant Protein D (SP-D) from bronchoalveolar lavage. Remarks: The epitope is localized in the lectin domain of SP-D.
    Clone
    VIF11
    Isotype
    IgG1
    Top Product
    Discover our top product SFTPD Primary Antibody
  • Application Notes
    Immunohistochemistry on Frozen Sections: 0.5 μg/mL (1/800) for Rat Cryosections and10-20 μg/mL (1/20-1/40) for Human Frozen Sections. Immunohistochemistry on Paraffin Sections: 10-20 μg/mL (1/20-1/40) for Rat paraffinsections (microwave pretreatment for antigen retrieval is recommended). Suggested Positive Control: Rat lung. Has been described to work in ELISA and Western blots.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore by adding 0.5 mL distilled water (= 0.4 mg/mL Stock Solution).
    Concentration
    0.4 mg/mL
    Buffer
    Stock solution contains PBS, pH 7.2 with 0.09 % Sodium Azide as preservative and 5 mg/mL BSA as stabilizer
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store at -20 °C.
  • Target
    SFTPD (Surfactant Protein D (SFTPD))
    Alternative Name
    SFTPD (SFTPD Products)
    Synonyms
    COLEC7 antibody, PSP-D antibody, SFTP4 antibody, SP-D antibody, BDE antibody, BDSD antibody, HOX4I antibody, SPD antibody, AI573415 antibody, Sftp4 antibody, surfactant protein D antibody, homeobox D13 antibody, surfactant associated protein D antibody, SFTPD antibody, HOXD13 antibody, Sftpd antibody, SP-D antibody
    Background
    Surfactant protein D is a Ca2+ -dependent carbohydrate binding protein with a similar structure as other C-type mammalian lectins such as conglutinin and SP-A. The molecular weight is 620 kD under non reducing conditions and 43 kD under reducing conditions. The function of SP-D is not clearly defined. SP-D enhances the production of oxygen radicals in alveolar macrophages and regulates some actions of SP-A, which is the most abundant surfactant protein. SP-D is synthesized and secreted by alveolar epithelial type II cells.Synonyms: COLEC7, Collectin-7, Lung surfactant protein D, PSP-D, PSPD, Pulmonary surfactant-associated protein D, SFTP4, SP-D
    Gene ID
    25350
    NCBI Accession
    NP_037010
    UniProt
    P35248
You are here:
Support