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Goat anti-Mouse IgG1 (Heavy Chain) Antibody (Atto 594) - Preadsorbed

WB, FLISA, FM Polyclonal IgG1 Atto 594
Catalog No. ABIN1118518
  • Target See all IgG1 products
    IgG1
    Binding Specificity
    • 41
    • 26
    • 17
    • 6
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 84
    • 25
    • 23
    • 7
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 1
    Mouse
    Host
    • 63
    • 39
    • 35
    • 10
    • 9
    • 6
    • 1
    Goat
    Clonality
    • 96
    • 54
    Polyclonal
    Conjugate
    • 44
    • 18
    • 18
    • 16
    • 11
    • 8
    • 7
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Atto 594
    Application
    • 76
    • 56
    • 51
    • 42
    • 31
    • 20
    • 18
    • 12
    • 11
    • 10
    • 10
    • 9
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG.
    Specificity was confirmed by ELISA at less than 0.5 % of target signal.
    Cross-Reactivity
    Mouse (Murine)
    Characteristics
    ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
    Purification
    Preadsorption: Solid phase absorption
    Immunogen

    Immunogen: Mouse IgG1 heavy chain

    Immunogen Type: Native Protein

    Isotype
    IgG1
    Labeling Ratio
    3.0
  • Application Notes

    Application Note: ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    FLISA Dilution: >1:20,000

    Western Blot Dilution: >1:10,000

    IF Microscopy Dilution: >1:5,000

    Comment

    The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 500 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid cycles of freezing and thawing.
    Product is photosensitive and should be protected from light.
    Storage
    RT,4 °C,-20 °C
    Storage Comment
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Expiry Date
    12 months
  • Target
    IgG1
    Abstract
    IgG1 Products
    Target Type
    Antibody
    Background

    Synonyms: Goat anti-mouse IgG1 antibody ATTO594 conjugation, goat anti-mouse IgG1 (gamma 1) ATTO 594 conjugated antibody

    Background: Anti-Mouse IgG1 ATTO 594 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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