CD209b Antigen (CD209B) antibody

Catalog No. ABIN111861

Product Details

Target: CD209b Antigen (CD209B)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: ER-TR9 antibody is a very useful marker for the identification of macrophage subpopulations present in the splenic marginal zone, lymph node medulla and other organs. In combination with MOMA-1 (Cat-Nr SM066P), the murine metallophilic macrophage marker, a detailed characterization of murine splenic marginal zone macrophages is obtained. ER-TR9 is also useful when studying phagocytosis of neutral polysaccharides since the antibody selectively inhibits uptake of these glycans by macrophages. The antigen recognized by ER-TR9 has recently been shown to be the Murine analogue of the Human DC-SIGN (Dendritic Cell - Specific ICAM-3 Grabbing Non Integrin), named SIGN-R1. Antigen Distribution Antigen Distribution on Isolated cells: The antigen is found on a subpopulation of phagocytic macrophages isolated from the spleen and showing acid phosphatase and moderate non-specific esterase activity. These phagocytes selectively ingest neutral polysaccharides such as Ficoll. Antigen Distribution on Tissue Sections: Subpopulation of resident macrophages in the splenic marginal zone which are in the proximity of a certain B cell subpopulation (μ+, d-). It is also found on a subpopulation of macrophages localized in the medullary sinuses and trabecular sinuses of lymph nodes. Furthermore, macrophage subpopulations in other organs, such as some connective tissue macrophages in the dermis, may also show ER-TR9 antigen expression. This antibody is specific for a Mouse subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.

Cross-Reactivity (Details): Species reactivity (tested):Mouse: Subpopulation of mature tissue macrophages present in the splenic marginal zone, lymph node medullary and trabecular sinuses.

Purification: Affinity Chromatography

Immunogen: Thymus cells. Remarks: The antigen is a Glutaraldehyde (0.05%) resistant protein expressed in the cytoplasm and on the cell surface.

Clone: ER-TR9

Isotype: IgM

Application Details

Application Notes: Immunohistchemistry on Frozen Sections: 0.5 μg/mL (1/600). Does not react on routinely processed Paraffin sections. Recommended Positive Control: Mouse spleen. Has been described to work in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water.

Concentration: 0.3 mg/mL

Buffer: Stock solution contains PBS, pH 7.2 with 0.1 % Kathon as a preservative

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

References

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Target Details

Target: CD209b Antigen (CD209B)

Alternative Name: CD209b / DC-SIGNR1 (CD209B Products)

Synonyms: 1810030I22Rik antibody, OtB7 antibody, SIGNR1 antibody, mSIGNR1 antibody, CD209b antigen antibody, Cd209b antibody

Background: Synonyms: CD209 antigen-like protein B, DC-SIGN-related protein 1, OtB7

Gene ID: 69165

NCBI Accession: NP_001032889

UniProt: Q8CJ91