S100A8 antibody (S100 Calcium Binding Protein A8)

Details for Product anti-S100A8 Antibody No. ABIN111890, Supplier: Log in to see
Antigen
  • EWWD
  • MRP8
  • WW
  • 60B8AG
  • CAGA
  • CFAG
  • CGLA
  • CP-10
  • L1Ag
  • MA387
  • MIF
  • NIF
  • P8
  • Mrp8
  • S100A8
  • 60B8Ag
  • AI323541
  • B8Ag
  • CFAg
  • Caga
  • p8
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 11
  • S100 calcium binding protein A8
  • S100 calcium binding protein A8 (calgranulin A)
  • ABCC11
  • S100A8
  • S100a8
Alternatives
anti-Human S100A8 antibody for Immunohistochemistry
Reactivity
Human
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Host
Mouse
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1
Clonality (Clone)
Monoclonal ()
Conjugate
This S100A8 antibody is un-conjugated
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11
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Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Options
Supplier
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Immunogen Cultured human monocytes.
Clone 8-5C2
Isotype IgG1
Specificity This Monoclonal 8-5C2 antibody reacts with Human MRP8 in stimulated monocytes and macrophages in late phase or chronic inflammation. The antigen is MRP8, the epitope is suspected in the central portion of the peptide. Antigen Distribution on Isolated Cells: The antigen is found in granulocytes and monocytes but not in other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stain positive. Antigen Distribution on Tissue Sections: MRP8 is found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP8 increasingly during the late stages of inflammation. A low MRP8 (and high MRP- 14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis or chronic rejection after allograft transplantation, MRP8 and MRP14 positive cells consist of different subpopulations.
No Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Species reactivity (tested):Human. The antibody reacts with Bovine spleen and shows a diffuse reaction with Swine spleen.
Purification Affinity Chromatography
Alternative Name S100A8 / Calgranulin-A / MRP8 (S100A8 Antibody Abstract)
Background Monoclonal antibody 8-5C2 identifies MRP8 (also named S100A8 or Calgranulin A), the Ca2+-binding light subunit of the inflammatory L-1 protein complex. MRP8 forms Ca2+ dependent dimers or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8. The antibody is useful in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
Gene ID 9606
UniProt P05109
Research Area Cell Cycle
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity
Application Notes ELISA. Immunohistochemistry on Frozen Sections: 1-2 μg/mL (1/100-1/200). Immunohistochemistry on Paraffin Sections: 5-10 μg/mL (1/20-1/50) (No pretreatment forantigen retrieval necessary). Microwave treatment in 0.01M Citrate, pH 6.0, may enhance the reactivity. Suggested Positive Control: Human tonsil. Has been described to work in Dot Blots and not in FACS.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Protocol with frozen, ice-cold acetone-fixed sections: (The whole procedure is performed at room temperature)1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber
Restrictions For Research Use only
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer Stock Solution contains PBS, pH 7.2, 5 mg/mL BSA, 0.05 % Kathon
Preservative Kathon CG
Precaution of Use This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (IHC) image for anti-S100 Calcium Binding Protein A8 (S100A8) antibody (ABIN111890) Immunohistochemistry on human tonsil paraffin sections using S100A8 antibody clone 8-...
Product cited in: Wong, Zhou, Li, Tong, Zhao, Li, Yu, Koh, Beuerman: "Proteomic profiling of inflammatory signaling molecules in the tears of patients on chronic glaucoma medication." in: Investigative ophthalmology & visual science, Vol. 52, Issue 10, pp. 7385-91, 2011 (PubMed).

Mortezai, Harder, Schnabel, Moors, Gauly, Schlüter, Wagener, Buck: "Tandem affinity depletion: a combination of affinity fractionation and immunoaffinity depletion allows the detection of low-abundance components in the complex proteomes of body fluids." in: Journal of proteome research, Vol. 9, Issue 12, pp. 6126-34, 2010 (PubMed).

Rosenberger, Thorey, Werner, Boukamp: "A novel regulator of telomerase. S100A8 mediates differentiation-dependent and calcium-induced inhibition of telomerase activity in the human epidermal keratinocyte line HaCaT." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6126-35, 2007 (PubMed).

Background publications Wen, Xu, Mais, Goldman, McDonnell: "The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8356-64, 1994 (PubMed).

Teigelkamp, Bhardwaj, Roth, Meinardus-Hager, Karas, Sorg: "Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14." in: The Journal of biological chemistry, Vol. 266, Issue 20, pp. 13462-7, 1991 (PubMed).

Delabie, de Wolf-Peeters, van den Oord, Desmet: "Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study." in: Clinical and experimental immunology, Vol. 81, Issue 1, pp. 123-6, 1990 (PubMed).

Mues, Brisse, Zwadlo, Themann, Bender, Sorg: "Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis." in: European heart journal, Vol. 11, Issue 7, pp. 619-27, 1990 (PubMed).

Brandtzaeg, Jones, Flavell, Fagerhol: "Mac 387 antibody and detection of formalin resistant myelomonocytic L1 antigen." in: Journal of clinical pathology, Vol. 41, Issue 9, pp. 963-70, 1989 (PubMed).

Murao, Collart, Huberman: "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." in: The Journal of biological chemistry, Vol. 264, Issue 14, pp. 8356-60, 1989 (PubMed).

Zwadlo, Brüggen, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues." in: Clinical and experimental immunology, Vol. 72, Issue 3, pp. 510-5, 1988 (PubMed).

Odink, Cerletti, Brüggen, Clerc, Tarcsay, Zwadlo, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." in: Nature, Vol. 330, Issue 6143, pp. 80-2, 1987 (PubMed).

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