S100A8 antibody (S100 Calcium Binding Protein A8) (C-Term) (Biotin)

Details for Product anti-S100A8 Antibody No. ABIN111893, Supplier: Log in to see
Antigen
  • EWWD
  • MRP8
  • WW
  • 60B8AG
  • CAGA
  • CFAG
  • CGLA
  • CP-10
  • L1Ag
  • MA387
  • MIF
  • NIF
  • P8
  • Mrp8
  • S100A8
  • 60B8Ag
  • AI323541
  • B8Ag
  • CFAg
  • Caga
  • p8
  • ATP-binding cassette, sub-family C (CFTR/MRP), member 11
  • S100 calcium binding protein A8
  • S100 calcium binding protein A8 (calgranulin A)
  • ABCC11
  • S100A8
  • S100a8
Alternatives
anti-Rat (Rattus) S100A8 antibody for Dot Blot
Epitope
C-Term
27
24
13
9
9
7
5
4
3
2
2
1
1
1
1
1
Reactivity
Human, Pig (Porcine), Rat (Rattus)
172
70
45
10
8
5
4
4
3
2
2
1
Host
Mouse
113
84
29
22
4
1
Clonality (Clone)
Monoclonal ()
Conjugate
This S100A8 antibody is conjugated to Biotin
25
22
11
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
187
114
63
62
46
26
24
15
8
7
7
7
7
1
1
1
Options
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Immunogen Cultured Human Monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the N- or C-terminal portion of the peptide.
Clone S13-67
Isotype IgG1
Specificity S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8 kDa S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).
Cross-Reactivity (Details) Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages inlate phase or chronic inflammation.Rat, Cow, Pig (TNF-alpha/IL-1 alpha induced inflammation of the skin).
Purification Affinity Chromatography
Alternative Name S100A8 / Calgranulin-A / MRP8 (S100A8 Antibody Abstract)
Background MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
Gene ID 9606
UniProt P05109
Research Area Cell Cycle
Pathways Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity
Application Notes Flow cytometry. Immunohistochemistry on: Frozen sections: 1 μg/mL (1: 200)Paraffin sections: 5-10 μg/mL (1: 20-1: 50), no pretreatment for antigen retrieval necessary.
Restrictions For Research Use only
Reconstitution Restore in 0.5 mL distilled water to make stock solution.
Concentration 0.2 mg/mL
Buffer PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Background publications Kumar, Yang, Bilson, Thliveris, Cooke, Geczy: "Dimeric S100A8 in human neutrophils is diminished after phagocytosis." in: Journal of leukocyte biology, Vol. 70, Issue 1, pp. 59-64, 2001 (PubMed).

Wen, Xu, Mais, Goldman, McDonnell: "The A and B isoforms of the human progesterone receptor operate through distinct signaling pathways within target cells." in: Molecular and cellular biology, Vol. 14, Issue 12, pp. 8356-64, 1994 (PubMed).

Teigelkamp, Bhardwaj, Roth, Meinardus-Hager, Karas, Sorg: "Calcium-dependent complex assembly of the myeloic differentiation proteins MRP-8 and MRP-14." in: The Journal of biological chemistry, Vol. 266, Issue 20, pp. 13462-7, 1991 (PubMed).

Delabie, de Wolf-Peeters, van den Oord, Desmet: "Differential expression of the calcium-binding proteins MRP8 and MRP14 in granulomatous conditions: an immunohistochemical study." in: Clinical and experimental immunology, Vol. 81, Issue 1, pp. 123-6, 1990 (PubMed).

Mues, Brisse, Zwadlo, Themann, Bender, Sorg: "Phenotyping of macrophages with monoclonal antibodies in endomyocardial biopsies as a new approach to diagnosis of myocarditis." in: European heart journal, Vol. 11, Issue 7, pp. 619-27, 1990 (PubMed).

Hogg, Allen, Edgeworth: "Monoclonal antibody 5.5 reacts with p8,14, a myeloid molecule associated with some vascular endothelium." in: European journal of immunology, Vol. 19, Issue 6, pp. 1053-61, 1989 (PubMed).

Murao, Collart, Huberman: "A protein containing the cystic fibrosis antigen is an inhibitor of protein kinases." in: The Journal of biological chemistry, Vol. 264, Issue 14, pp. 8356-60, 1989 (PubMed).

Zwadlo, Brüggen, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins associated with specific stages of myeloid cell differentiation are expressed by subsets of macrophages in inflammatory tissues." in: Clinical and experimental immunology, Vol. 72, Issue 3, pp. 510-5, 1988 (PubMed).

Odink, Cerletti, Brüggen, Clerc, Tarcsay, Zwadlo, Gerhards, Schlegel, Sorg: "Two calcium-binding proteins in infiltrate macrophages of rheumatoid arthritis." in: Nature, Vol. 330, Issue 6143, pp. 80-2, 1987 (PubMed).