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S100A8 antibody (C-Term) (Biotin)

S100A8 Reactivity: Human, Rat, Pig WB, FACS, IHC (p), IHC (fro), EIA Host: Mouse Monoclonal S13-67 Biotin
Catalog No. ABIN111893
  • Target See all S100A8 Antibodies
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Binding Specificity
    • 18
    • 18
    • 16
    • 9
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 84
    • 51
    • 32
    • 14
    • 9
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 4
    • 4
    Human, Rat, Pig
    Host
    • 81
    • 37
    • 3
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 86
    • 40
    Monoclonal
    Conjugate
    • 65
    • 20
    • 16
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This S100A8 antibody is conjugated to Biotin
    Application
    • 74
    • 47
    • 43
    • 30
    • 16
    • 15
    • 14
    • 14
    • 13
    • 12
    • 7
    • 6
    • 5
    • 5
    • 3
    • 3
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)
    Specificity
    S 13.67 identifies the Ca2+-binding light subunit of the inflammatory L-1 protein complex, also called S100A8 or Calgranulin A. S 13.67 is useful for the identification of the 8 kDa S100A8 subunit in various immunological techniques. Histological and serological data indicate that MRP8 is associated with chronic stages of inflammatory diseases. S13.67 detects a very distinct, as yet not characterized epitope of MRP8 (different from the 8-5C2 epitope). Antigen Distribution Isolated cells: The antigen is found in granulocytes and monocytes. It is absent from other blood cells. In cultured monocytes, maximum MRP8 is expressed after 3-4 days. Myeloid leukaemia stains positively. Tissue Sections: In the tissue, MRP-8 is only found in a distinct subpopulation of inflammatory perivascular infiltrates of the myelo-monocytic lineage. Macrophages synthesise MRP-8 increasingly during the late stages of inflammation. A low MRP-8 (and high MRP-14) expression by macrophages was also reported in granulomatous diseases such as tuberculosis and sarcoidis. In non-granulomatous chronic inflammatory diseases such as chronic rheumatoid arthritis MRP8 and MRP14 positive cells consist of different subpopulations. During early inflammation endothelial cells are also positive with MRP8 / MRP14(TNF-alpha/IL-1 alpha induced inflammation of the skin).
    Cross-Reactivity (Details)
    Species reactivity (tested):Human: MRP8 in stimulated monocytes and macrophages inlate phase or chronic inflammation.Rat, Cow, Pig (TNF-alpha/IL-1 alpha induced inflammation of the skin).
    Purification
    Affinity Chromatography
    Immunogen
    Cultured Human Monocytes. Remarks: The antigen is MRP8, the epitope is suspected in the N- or C-terminal portion of the peptide.
    Clone
    S13-67
    Isotype
    IgG1
    Top Product
    Discover our top product S100A8 Primary Antibody
  • Application Notes
    Flow cytometry. Immunohistochemistry on: Frozen sections: 1 μg/mL (1: 200)Paraffin sections: 5-10 μg/mL (1: 20-1: 50), no pretreatment for antigen retrieval necessary.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 0.5 mL distilled water to make stock solution.
    Concentration
    0.2 mg/mL
    Buffer
    PBS, pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Target
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Alternative Name
    S100A8 / Calgranulin-A / MRP8 (S100A8 Products)
    Synonyms
    60B8AG antibody, CAGA antibody, CFAG antibody, CGLA antibody, CP-10 antibody, L1Ag antibody, MA387 antibody, MIF antibody, MRP8 antibody, NIF antibody, P8 antibody, Mrp8 antibody, S100A8 antibody, 60B8Ag antibody, AI323541 antibody, B8Ag antibody, CFAg antibody, Caga antibody, p8 antibody, MRP-8 antibody, S100 calcium binding protein A8 antibody, S100 calcium binding protein A8 (calgranulin A) antibody, S100A8 antibody, S100a8 antibody
    Background
    MRP8 is the Ca2+-binding light subunit of Calprotectin. MRP8 forms Ca2+-dependent or complexes with MRP14 (S100A9, Calgranulin B). It also forms disulfide-linked homodimers under the influence of hypochlorite, a process thought to abrogate the chemotactic property of MRP8.Synonyms: CAGA, CFAG, Calprotectin L1L subunit, Cystic fibrosis antigen, Leukocyte L1 complex light chain, MRP-8, Migration inhibitory factor-related protein 8, S100 calcium-binding protein A8, S100-A8, Urinary stone protein band A
    Gene ID
    9606
    UniProt
    P05109
    Pathways
    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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