Integrin beta 2 antibody (Activation Site)

Catalog No. ABIN111902

Product Details anti-Integrin beta 2 Antibody

Target: Integrin beta 2 (ITGB2)

Binding Specificity: Activation Site

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)

Specificity: The antibody recognizes the epitope exposed on free human CD18 chains unassociated with CD11 as well as on high-affinity state of LFA-1. CD18 (integrin b2 subunit, b2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

Cross-Reactivity (Details): Species reactivity (tested):Human. Negative with Porcine.

Purification: Protein A Affinity Chromatography

Immunogen: Peripheral blood mononuclear cells.

Clone: MEM-148

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: The antibody is excellent marker of activated myeloid cells. Immunoprecipitation. Western Blotting (Non Reducing Conditions). Functional Application: The antibody induces homotypic cell aggregation and high-affinityLFA-1 conformation in resting leukocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, with 15 mM Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Integrin beta 2 antibody (ABIN111902)

Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2 (ITGB2 Products)

Synonyms: CD18 antibody, LAD antibody, LCAMB antibody, LFA-1 antibody, MAC-1 antibody, MF17 antibody, MFI7 antibody, 2E6 antibody, AI528527 antibody, Cd18 antibody, Lfa1 antibody, ITGB2 antibody, itgb2 antibody, itgb2-a antibody, itgb2-b antibody, integrin subunit beta 2 antibody, integrin beta 2 antibody, integrin subunit beta 2 S homeolog antibody, ITGB2 antibody, Itgb2 antibody, itgb2.S antibody

Background: CD18, integrin b2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (b2) integrins: aLb2 (CD11a/CD18, LFA-1), aMb2 (CD11b/CD18, Mac-1, CR3), aXb2 (CD11c/CD18) and aDb2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 3689

NCBI Accession: NP_000202

UniProt: P05107

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation