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ICAM1 antibody

ICAM1 Reactivity: Human WB, FACS, IHC (p), IHC (fro) Host: Mouse Monoclonal 1304 unconjugated
Catalog No. ABIN111934
  • Target See all ICAM1 Antibodies
    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
    Reactivity
    • 209
    • 80
    • 38
    • 7
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    Human
    Host
    • 120
    • 108
    • 27
    • 4
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 140
    • 119
    • 1
    Monoclonal
    Conjugate
    • 121
    • 26
    • 23
    • 18
    • 9
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ICAM1 antibody is un-conjugated
    Application
    • 166
    • 150
    • 62
    • 61
    • 45
    • 40
    • 35
    • 35
    • 28
    • 28
    • 25
    • 20
    • 12
    • 10
    • 6
    • 4
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Protein A Chromatography
    Immunogen
    Cultured Human endothelial cells.
    Clone
    1304
    Isotype
    IgG2a
    Top Product
    Discover our top product ICAM1 Primary Antibody
  • Application Notes
    Suitable for Immunohistochemistry (IHC), FACS and Western Blots. Recommended Dilutions for IHC: Frozen sections: 0.25 μg/mL (1/800). Paraffin sections: 2 μg/mL (1/100), Proteinase K pretreatment for antigen retrieval isrecommended. Suggested positive control: Human tonsil.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalumProtocol with formalin-fixed, paraffin-embedded sections: The whole procedure is performed at room temperature1. Deparaffinize and rehydrate tissue section2. Incubate the tissue section with proteinase K for 7min. 3. Wash in distilled water4. Block endogenous peroxidase5. Wash in PBS
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water.
    Concentration
    0.2 mg/mL
    Buffer
    PBS pH 7.2 with 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))
    Alternative Name
    CD54 / ICAM1 (ICAM1 Products)
    Synonyms
    BB2 antibody, CD54 antibody, P3.58 antibody, Icam-1 antibody, Ly-47 antibody, MALA-2 antibody, ICAM antibody, ICAM-1 antibody, ICAM1 antibody, intercellular adhesion molecule 1 antibody, intercellular adhesion molecule-1 antibody, ICAM1 antibody, Icam1 antibody, ICAM-1 antibody
    Target Type
    Viral Protein
    Background
    ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
    Gene ID
    9606
    UniProt
    P05362
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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