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ICAM-1 antibody (Intercellular Adhesion Molecule 1) (Biotin)

Details for Product anti-ICAM1 Antibody No. ABIN111935, Supplier: Log in to see
Antigen
  • BB2
  • CD54
  • P3.58
  • Icam-1
  • Ly-47
  • MALA-2
  • ICAM
  • ICAM-1
  • ICAM1
  • intercellular adhesion molecule 1
  • intercellular adhesion molecule-1
  • intercellular adhesion molecule-1 precursor
  • ICAM1
  • Icam1
  • ICAM-1
Alternatives
anti-Human ICAM-1 antibody for Flow Cytometry
Reactivity
Human
727
172
155
28
13
9
3
1
1
1
1
Host
Mouse
565
261
83
8
8
7
4
2
Clonality (Clone)
Monoclonal ()
Conjugate
This ICAM-1 antibody is conjugated to Biotin
79
77
76
21
20
19
14
13
11
11
10
9
9
9
9
9
6
6
6
6
5
5
4
4
4
4
4
4
4
3
3
2
2
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
557
401
267
200
176
151
141
74
53
53
34
18
14
14
12
10
9
8
7
6
6
6
5
4
4
3
2
2
2
2
2
2
1
1
1
1
1
1
Options
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Immunogen Cultured Human endothelial cells
Clone 1304
Isotype IgG2a
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Protein A Chromatography.
Alternative Name CD54 / ICAM1 (ICAM1 Antibody Abstract)
Background ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.Synonyms: ICAM-1, Intercellular adhesion molecule 1, Major Group Rhinovirus Receptor
Gene ID 9606
UniProt P05362
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity
Application Notes Immunohistochemistry on Frozen Sections: 0.25 μg/mL (1/800). Immunohistochemistry on Paraffin Sections: 2 μg/mL (1/100). Proteinase K pretreatmentfor antigen retrieval is recommended. Suggested Positive Control: Human tonsil. Has been described to work in FACS and Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Reconstitution Restore with 0.5 mL distilled water.
Concentration 0.2 mg/mL
Buffer Stock Solution contains PBS, pH 7.2 with 0.01 % Thimerosal as preservative and 1 % BSA as stabilizer.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Store the antibody undiluted Prior to and following reconstitution at 2-8 °C. DO NOT FREEZE.
Background publications Albelda, Buck: "Integrins and other cell adhesion molecules." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 4, Issue 11, pp. 2868-80, 1990 (PubMed).

Freudenthal, Steinman: "The distinct surface of human blood dendritic cells, as observed after an improved isolation method." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 19, pp. 7698-702, 1990 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Rothlein, Dustin, Marlin, Springer: "A human intercellular adhesion molecule (ICAM-1) distinct from LFA-1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 4, pp. 1270-4, 1986 (PubMed).