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TNFRSF1A antibody

TNFRSF1A Reactivity: Human WB, FACS, IHC (p), IHC (fro) Host: Mouse Monoclonal htr9 unconjugated
Catalog No. ABIN111937
  • Target See all TNFRSF1A Antibodies
    TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
    Reactivity
    • 144
    • 80
    • 46
    • 7
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 125
    • 41
    • 9
    • 5
    • 2
    • 2
    • 1
    Mouse
    Clonality
    • 130
    • 54
    Monoclonal
    Conjugate
    • 90
    • 22
    • 19
    • 12
    • 9
    • 7
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TNFRSF1A antibody is un-conjugated
    Application
    • 132
    • 83
    • 66
    • 61
    • 50
    • 30
    • 23
    • 22
    • 21
    • 18
    • 13
    • 13
    • 11
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purification
    Immunoaffinity Chromatography
    Immunogen
    Partially purified preparation of TNF binding protein from HL-60 cells.
    Clone
    Htr9
    Isotype
    IgG1
    Top Product
    Discover our top product TNFRSF1A Primary Antibody
  • Application Notes
    htr 9 is useful for studying biological effects of TNF-receptor p55 in vitro. The antibodyinhibits the binding of radiolabelled TNF to human cells expressing the p55 TNF receptor. Inorder to obtain complete inhibition of TNF binding to the cell surface, 10 μg/mL of htr-9 arerequired. htr 9 itself may have an agonistic effect in assays measuring cytotoxicity,
    Protocol
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 0.5 mL distilled water.
    Concentration
    0.4 mg/mL
    Buffer
    PBS, pH 7.2, 0.05 % Kathon CG, 5 mg/mL BSA
    Preservative
    Kathon CG
    Precaution of Use
    This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 4-8 °C for one month or at -20 °C for longer.
  • Target
    TNFRSF1A (Tumor Necrosis Factor Receptor Superfamily, Member 1A (TNFRSF1A))
    Alternative Name
    CD120a / TNFR1 (TNFRSF1A Products)
    Synonyms
    CD120a antibody, FPF antibody, MS5 antibody, TBP1 antibody, TNF-R antibody, TNF-R-I antibody, TNF-R55 antibody, TNFAR antibody, TNFR1 antibody, TNFR1-d2 antibody, TNFR55 antibody, TNFR60 antibody, p55 antibody, p55-R antibody, p60 antibody, TNF-R1 antibody, TNF-alphaR1 antibody, TNFRI antibody, TNFRp55 antibody, TNFalpha-R1 antibody, Tnfr-2 antibody, Tnfr1 antibody, fpf antibody, tbp1 antibody, p55-r antibody, tnf-r antibody, tnfar antibody, tnfr1 antibody, cd120a antibody, tnfr55 antibody, tnfr60 antibody, xtnfr1 antibody, wu:fj65b10 antibody, zgc:85914 antibody, TNFR I antibody, TNFRSF1A antibody, TNFA antibody, cTNF antibody, TNF receptor superfamily member 1A antibody, tumor necrosis factor receptor superfamily, member 1a antibody, TNF receptor superfamily member 1A S homeolog antibody, tumor necrosis factor antibody, TNFRSF1A antibody, Tnfrsf1a antibody, tnfrsf1a.S antibody, tnfrsf1a antibody, TNF antibody
    Background
    Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF Receptor superfamily along with FAS antigen and CD40. TNF Receptors I and II are 55 and 75 kDa members, respectively, of a family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 41BB, which are characterized by cysteine rich motifs in the extracellular domain. While TNF Receptor I and TNF Receptor II share 28 % sequence homology in the extracellular domains, their intracellular domains lack sequence homology, suggesting that they differ in their internal signal transduction pathways. TNF Receptor I contains an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein), and subsequent interactions with FADD. TNF Receptor I can also activate the transcription factor NFkB via TRAF2 (TNF Receptor associated factor 2). The cytoplasmic domain of TNF Receptor I can directly interact with Jak kinase, thereby activating the JAK/STAT signal transduction cascade. TNF Receptor I is expressed by virtually all nucleated mammalian cells, including hepatocytes, monocytes and neutrophils, cardiac muscle cells, endothelial cells, and CD34 + hematopoietic progenitors. Both TNF alpha and TNF beta bind to TNF Receptor I.Synonyms: TNF-R1, TNF-RI, TNFR-I, Tnfrsf1a, Tumor necrosis factor receptor 1, Tumor necrosis factor receptor superfamily member 1A, Tumor necrosis factor receptor type I, p55, p60
    Gene ID
    9606
    UniProt
    P19438
    Pathways
    NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, Hepatitis C, Ubiquitin Proteasome Pathway
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