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TNFRSF1B antibody (Biotin)

TNFRSF1B Reactivity: Human WB, FACS, IHC (fro) Host: Mouse Monoclonal utr1 Biotin
Catalog No. ABIN111941
  • Target See all TNFRSF1B Antibodies
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Reactivity
    • 81
    • 58
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Human
    Host
    • 62
    • 27
    • 11
    • 4
    • 1
    • 1
    • 1
    Mouse
    Clonality
    • 55
    • 52
    Monoclonal
    Conjugate
    • 66
    • 16
    • 9
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This TNFRSF1B antibody is conjugated to Biotin
    Application
    • 68
    • 36
    • 32
    • 31
    • 28
    • 23
    • 19
    • 17
    • 14
    • 9
    • 7
    • 6
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Affinity Chromatography
    Immunogen
    Partially purified preparations of TNF binding proteins
    Clone
    Utr1
    Isotype
    IgG1
    Top Product
    Discover our top product TNFRSF1B Primary Antibody
  • Application Notes
    Immunohistochemistry on Frozen Sections: 1 μg/mL (1/400) andImmunohistochemistry on Paraffin Sections: 10 μg/mL (1/40). Pretreatment not necessary. Recommended Positive Control: Human tonsil. Has also been described to work in FACS, Western Blots and Immunoprecipitation.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS 11. Counterstain with Mayer’s hemalum.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore by adding 0.5 mL distilled water.
    Concentration
    0.4 mg/mL
    Buffer
    PBS, pH 7.2, containing 10 mg/mL BSA as a stabilizer and 0.01 % Thimerosal as a preservative
    Preservative
    Thimerosal (Merthiolate)
    Precaution of Use
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted Prior to and After reconstitution at 2-8 °C. DO NOT FREEZE!.
  • Target
    TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))
    Alternative Name
    CD120b / TNFR2 (TNFRSF1B Products)
    Synonyms
    CD120b antibody, TBPII antibody, TNF-R-II antibody, TNF-R75 antibody, TNFBR antibody, TNFR1B antibody, TNFR2 antibody, TNFR80 antibody, p75 antibody, p75TNFR antibody, TNF-RII/TNFRSF1B antibody, TNFR-II antibody, TNFRSF1B antibody, TNF-R2 antibody, TNF-alphaR2 antibody, TNFRII antibody, TNFalpha-R2 antibody, Tnfr-1 antibody, Tnfr2 antibody, rank antibody, zgc:163064 antibody, TNF receptor superfamily member 1B antibody, tumor necrosis factor receptor superfamily, member 1b antibody, tumor necrosis factor receptor superfamily, member 1B antibody, TNFRSF1B antibody, Tnfrsf1b antibody, tnfrsf1b antibody
    Background
    Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.Synonyms: TNF-R2, TNFBR, TNFRSF1B, Tumor necrosis factor receptor 2, Tumor necrosis factor receptor superfamily member 1B, Tumor necrosis factor receptor type II, p80 TNF-alpha receptor
    Gene ID
    9606
    UniProt
    P20333
    Pathways
    NF-kappaB Signaling, Apoptosis, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway
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