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NEFH antibody

NEFH Reactivity: Pig WB, IHC (p), IF, IHC (fro) Host: Mouse Monoclonal NF-01 unconjugated
Catalog No. ABIN111968
  • Target See all NEFH Antibodies
    NEFH (Neurofilament, Heavy Polypeptide (NEFH))
    Reactivity
    • 109
    • 82
    • 64
    • 48
    • 28
    • 28
    • 12
    • 12
    • 9
    • 9
    • 9
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    Pig
    Host
    • 67
    • 56
    • 10
    Mouse
    Clonality
    • 83
    • 50
    Monoclonal
    Conjugate
    • 90
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This NEFH antibody is un-conjugated
    Application
    • 90
    • 44
    • 37
    • 29
    • 27
    • 24
    • 24
    • 16
    • 14
    • 13
    • 13
    • 10
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Purification
    Protein-A affinity chromatography
    Immunogen
    Pellet of porcine brain cold-stable proteins after depolymerization of microtubules.
    Clone
    NF-01
    Isotype
    IgG1
    Top Product
    Discover our top product NEFH Primary Antibody
  • Application Notes
    Immunohistochemistry on frozen sections and paraffin sections (0.2 μg/mL). Immunocytochemistry. Western blot.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    PBS, pH ~7.4, 15 mM Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    NEFH (Neurofilament, Heavy Polypeptide (NEFH))
    Alternative Name
    Neurofilament H (200 kD) (NEFH Products)
    Synonyms
    NF-H antibody, NF200 antibody, Nfh antibody, mKIAA0845 antibody, NFH antibody, NEFH antibody, neurofilament heavy polypeptide antibody, neurofilament, heavy polypeptide antibody, neurofilament heavy antibody, MCYG_06231 antibody, Nefh antibody, NEFH antibody
    Background
    Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusively in neuronal cells, and in those cells most prominently in large axons, the longest cell protrusions known in vertebrates. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights (NF-H - heavy chain, NF-M - medium chain and NF-L - light chain). The three NF subunits share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their N-termini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm. The protein segment on the C-terminal side of the human NF-H rod is uniquely long (more than 600 AA) compared to other IF proteins and is highly charged (> 24 % Glu, > 25 % Lys), rich in proline (> 12 %) and improverished in cysteine, methionine and aromatic AA. Its most remarkable feature is a repetitive sequence that covers more than half its lenght and includes the sekvence motif Lys-Ser-Pro (KSP) greater than 40 times. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin.Synonyms: (Neuronal Marker), 200 kDa neurofilament protein, KIAA0845, NEFH, NF-H, NFH, Neurofilament triplet H protein, heavy polypeptide
    Gene ID
    9823
    UniProt
    P12037
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