Carbohydrate Antigen 19-9 (CA19-9) antibody

Antigen: Carbohydrate Antigen 19-9 (CA19-9)

Clonality: Monoclonal (1025-63-7)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN111972

Additional Information

Characteristics: Synonyms: CA19-9, Sialyl Lewis a, Sialosyl Lewis a

Alternative name: CA 19-9

Isotype: IgM

Clone: 1025-63-7

Description: Mucin glucoprotein is a sialyl Lewisa structure which is synthesized from type 1 bloodgroup precursor chains and is present in individuals expressing the Lewisa and/or Lewisbblood group antigens. In normal tissues, sialyl Lewisa antigen is present in ductalepithelium of the breast, kidney, salivary gland, and sweat glands. Its expression is greatlyenhanced in serum as well as in the majority of tumor cells in gastrointestinal (GI)carcinomas.

Specificity: The antibody 1025. 63. 7 can be used as a tumor marker for pancreas and colon carcinomaon frozen tissue sections and in serum except for patients with Le O blood groupdeterminant. It recognizes SLea and to a lesser degree SLex. The antigen is sensitive to formaldehyde fixation and paraffin embedding.

Application Details

Protocol: Protocol with frozen, ice-cold acetone-fixed sections: The whole procedure is performed at room temperature1. Wash in PBS2. Block endogenous peroxidase3. Wash in PBS4. Block with 10% normal goat serum in PBS for 30min. in a humid chamber5. Incubate with primary antibody (dilution see datasheet) for 1h in a humid chamber6. Wash in PBS7. Incubate with secondary antibody (peroxidase-conjugated goat anti mouse IgG+IgM(H+L) minimal-cross reaction to human) for 1h in a humid chamber8. Wash in PBS9. Incubate with AEC substrate (3-amino-9-ethylcarbazol) for 12min. 10. Wash in PBS11. Counterstain with Mayer's hemalum

Application Notes: Suitable for Immunohistochemistry on: Frozen sections: 1 ug/ml (1/100)Paraffin sections: 2 ug/ml (1/50), pretreatment not necessary. Suggested positive control: Human tonsil. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.1 mg/ml

Buffer: PBS, pH 7.2 with 10 mg/ml bovine serum albumin (BSA) as a stabilizer and0.01% Thimerosal as a preservative.Reconstitution: Restore with 0.5 ml distilled water.

Storage: Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Do not freeze working dilutionsAvoid repeated freezing and thawing. Shelf life: One year from despatch.

Research Area: Cancer, Cell/Tissue Markers, Blood Components

Restrictions: For Research Use only