CD31 antibody (Biotin)

Catalog No. ABIN111979

Product Details anti-CD31 Antibody (Biotin)

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD31 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography.

Immunogen: Mouse macrophage precursor cells. The antigen is a glutaraldehyde (0.05%) and paraformaldehyde (1%) resistant 140kD surface glycoprotein (reducing and non reducing conditions). The antigen expression closely follows that of CD34.

Clone: ER-MP12

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on acetone fixed Frozen Sections (1/1600 = 0.4 μg/mL)and Formalin Fixed Paraffin Sections (1/40 = 15 μg/mL). Antigen Retrieval: Proteinase K. Suggested Positive Control: Mouse spleen. Has been described to work in FACS analysis: 4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 0.5 mL distilled water (0.6 mg/mL stock solution).

Concentration: 0.6 mg/mL

Buffer: PBS, pH 7.2 containing 5 mg/mL BSA as a stabilizer and 0.05 % Kathon as a preservative.

Preservative: Kathon CG

Precaution of Use: This product contains Kathon CG a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

References for anti-CD31 antibody (Biotin) (ABIN111979)

Mateos, Landeira-Abia, Fafián-Labora, Fernández-Pernas, Lesende-Rodríguez, Fernández-Puente, Fernández-Moreno, Delmiro, Martín, Blanco, Arufe: "iTRAQ-based analysis of progerin expression reveals mitochondrial dysfunction, reactive oxygen species accumulation and altered proteostasis." in: Stem cell research & therapy, Vol. 6, pp. 119, (2016) (PubMed).

Morikawa, Kajimura, Nakamura, Hishiki, Nakanishi, Yukutake, Nagahata, Ishikawa, Hattori, Takenouchi, Takahashi, Ishii, Matsubara, Kabe, Uchiyama, Nagata, Gadalla, Snyder, Suematsu: "Hypoxic regulation of the cerebral microcirculation is mediated by a carbon monoxide-sensitive hydrogen sulfide pathway." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 4, pp. 1293-8, (2012) (PubMed).

Sakamoto, Geisel, Kim, Wyatt, Sellers, Smiley, Cooper, Russell, Rhoades: "Fibrinogen regulates the cytotoxicity of mycobacterial trehalose dimycolate but is not required for cell recruitment, cytokine response, or control of mycobacterial infection." in: Infection and immunity, Vol. 78, Issue 3, pp. 1004-11, (2010) (PubMed).

Larghero, Venè, Minghelli, Travaini, Morini, Ferrari, Pfeffer, Noonan, Albini, Benelli: "Biological assays and genomic analysis reveal lipoic acid modulation of endothelial cell behavior and gene expression." in: Carcinogenesis, Vol. 28, Issue 5, pp. 1008-20, (2007) (PubMed).

Li, Sainson, Shi, Leek, Harrington, Preusser, Biswas, Turley, Heikamp, Hainfellner, Harris: "Delta-like 4 Notch ligand regulates tumor angiogenesis, improves tumor vascular function, and promotes tumor growth in vivo." in: Cancer research, Vol. 67, Issue 23, pp. 11244-53, (2007) (PubMed).

Burbaeva, Boksha, Tereshkina, Savushkina, Starodubtseva, Turishcheva, Mukaetova-Ladinska: "Systemic neurochemical alterations in schizophrenic brain: glutamate metabolism in focus." in: Neurochemical research, Vol. 32, Issue 9, pp. 1434-44, (2007) (PubMed).

Li, Otsu, Murphy, Raymond: "Developmental decrease in NMDA receptor desensitization associated with shift to synapse and interaction with postsynaptic density-95." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 23, Issue 35, pp. 11244-54, (2003) (PubMed).

Target Details for CD31

Target: CD31 (PECAM1) (Platelet/endothelial Cell Adhesion Molecule (PECAM1))

Alternative Name: CD31 / PECAM1 (PECAM1 Products)

Synonyms: CD31 antibody, CD31/EndoCAM antibody, GPIIA' antibody, PECA1 antibody, PECAM-1 antibody, endoCAM antibody, C85791 antibody, Cd31 antibody, Pecam antibody, platelet/endothelial cell adhesion molecule 1 antibody, platelet and endothelial cell adhesion molecule 1 antibody, pecam1 antibody, PECAM1 antibody, Pecam1 antibody

Background: Anti-PECAM-1 is a general marker for the detection of a major population of haematopoietic stem cells and is useful for the accumulation of early murine macrophage precursors from bone marrow cell suspensions by FACS. An even more precise characterization of such isolated and concentrated precursor cells is achieved when ER-MP12 is combined with other markers like ER-MP20 (anti Ly-6C) or ER-MP58. The ER-MP12 antigen is also expressed by endothelial cells.Synonyms: End °CAM, GPIIA', PECAM-1, Platelet endothelial cell adhesion molecule

Gene ID: 10090

UniProt: Q08481

Pathways: Regulation of Actin Filament Polymerization